(* The format of the table was revised on Apr.25.2003.)
| Cells(1), Cells(2), Mycoplasma test, Isozyme analysis, STR-PCR(1), STR-PCR(2), Freezing process, Others(1), Others(2), |
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Additional Information(1) |
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[ Lot No.: 112586: token/seed (JCRB0103) ] |
Date cell culture started:11/25/86 Concentration of cells in an ampule: 1.7x10^6 cell/ml Viability under microscope (%): 96% Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, AST,G6PD,LD,MD,MPI,NP,PEPB. Cell Identification: available RFLP data: Cell line was identified to be unique by the JCRB CellID system. [ Culture condition of this lot.]
Freezing medium: Culture medium with 5% DMSO. Passage method: 0.01% or 0.02% EDTA and 0.05% trypsin. Growth temperature: 37 C CO2 concentration: 5 % Cell No. at passage: split ratio=1/6 - 1/8 Additional comments: Viability was 95% prior to freezing. Viability was checked at 10/07/87. [ Additional Data ]
(Date downloaded: 2008-06-13) |
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[ Lot No.: 112087: distribution (JCRB0103) ] |
Date cell culture started:11/20/87 Concentration of cells in an ampule: 2x10^6 cell/ml Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, G6PD,LD,NP. [ Culture condition of this lot.]
Passage method: 0.01% or 0.02% EDTA and 0.05% trypsin. Growth temperature: 37 Cell No. at passage: split ratio:1/6 - 1/8 Additional comments: Viability was 95% prior to freezing. [ Additional Data ]
(Date downloaded: 2008-06-13) |
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[ Lot No.: 061792: distribution (JCRB0103) ] |
Cell culture was started without cloning from the previous stock. Date cell culture started:05/11/92 Concentration of cells in an ampule: 7.7x10^5 cell/ml Viability under microscope (%): 97% Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Anchorage dependency: Yes [ Culture condition of this lot.]
Passage method: Scraping or 0.02%EDTA-0.05%trypsin treatment. Suspend in 2 ml of the medium.EDTA-trypsin recomended. Growth temperature: 37C Cell No. at passage: 6x10^5 cells /ml [ Additional Data ]
(Date downloaded: 2008-06-13) |
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[ Lot No.: 082994: distribution (JCRB0103) ] |
Passage Number, P52 Cell culture was started without cloning from the previous stock. Date cell culture started:07/26/94 Concentration of cells in an ampule: 1.48x10^6 cell/ml Growth rate: 93.0 hr Viability under microscope (%): 98.2 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, G6PD(typeB),LD,NP. Anchorage dependency: Yes [ Culture condition of this lot.]
Passage method: Wash cells once with PBS(-) and treated with 0.1% trypsin and 0.01% EDTA for 13-16 min. at 37 C. Growth temperature: 37 C Cell No. at passage: 2-2.5x10^4 cells/sq.cm Memo: from lot.112586 by Okado. [ Additional Data ]
(Date downloaded: 2008-06-13) |
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[ Lot No.: 101196: distribution (JCRB0103) ] |
Date cell culture started:09/20/96 Tests for mycoplasma, bacteria, and fungi were negative. [ Culture condition of this lot.]
[ Additional Data ]
(Date downloaded: 2008-06-13) |
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