Passage Number, P2*
Date cell culture started:03/03/87
Concentration of cells in an ampule: 1.5x10^6 cell/ml
Viability under microscope (%): 95%
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, AST,G6PD,LD,MD,MPI,NP,PEPB.

Culture medium: RPMI1640 + FCS 10%
Passage method: Dilution.
Growth temperature: 37 C
Cell No. at passage: 10^5 cells/ml
Additional comments: Prepared from NIHS0028. Viability was 78% prior to freezing. Viability was checked at 07/31/90.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Passage Number, P4*
Date cell culture started:11/30/87
Concentration of cells in an ampule: 1.3x10^6 cell/ml
Viability under microscope (%): 84%
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, G6PD,LD,NP.

Culture medium: RPMI1640 + FCS 10%
Passage method: Dilution.
Growth temperature: 37
Cell No. at passage: 10^5 cells/ml
Additional comments: Viability was 66% prior to freeze. Viability of 84% was checked at 11/08/88.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Passage Number, P5*
Date cell culture started:08/14/90
Concentration of cells in an ampule: 1.1x10^6 cell/ml
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.

Culture medium: RPMI1640 + FCS 10%
Passage method: Dilution.
Growth temperature: 37
Cell No. at passage: 10^5 cells/ml
Additional comments: Viability was 84.8% prior to freez.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Passage Number, P7*
Date cell culture started:06/22/90
Concentration of cells in an ampule: 7.5x10^5 cell/ml
Viability under microscope (%): 83%
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.

Culture medium: RPMI1640 + FCS 10%
Passage method: Dilution.
Growth temperature: 37
Cell No. at passage: 10^5 cells/ml
Additional comments: Viability was checked at 08/22/90.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Cells are propagated from the stock, 030387
Passage Number, P9*
Cell culture was started without cloning from the previous stock.
Date cell culture started:07/12/94
Concentration of cells in an ampule: 5.7x10^6 cell/ml
Growth rate: 38.5 hrs
Viability under microscope (%): 71.9
Antibiotics used :free
Anchorage dependency: No
Cell Identification: available

Culture medium: RPMI1640 medium with 10% heat-inactivated fetal bovine serum (J.R.Scientific, DO10400)
Freezing medium: Culture medium with 5% DMSO.
Passage method: Simple dilution.
Growth temperature: 37 C
Cell No. at passage: 3-5x10^5 cells/ml
Memo: from lot.030387(token) by Ohnishi

[ Additional Data ]

Cells(2), Mycoplasma test, Isozyme analysis, STR-PCR(1), STR-PCR(2),

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(Date downloaded: 2008-06-13)

Passage Number, P8*

[ Additional Data ]

(Date downloaded: 2008-06-13)

Cells are propagated from the stock, 030387
Passage Number, P12*

[ Additional Data ]

(Date downloaded: 2008-06-13)