Passage Number, UNK
Date cell culture started:12/12/86
Concentration of cells in an ampule: 6x10^6 cell/ml
Viability under microscope (%): 43%
Sterility test has not completed yet.

Culture medium: RPMI1640 medium with 10% fetal calf serum
Passage method: Dilution.
Growth temperature: 37
Cell No. at passage: 10^5 cells/ml
Additional comments: Prepared by K.Kishi at Niigata Univ. Viability was checked at 01/14/87.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Passage Number, P2*
Date cell culture started:02/10/87
Concentration of cells in an ampule: 5x10^5 cell/ml
Viability under microscope (%): 74%
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, AST,G6PD,LD,MD,MPI,NP,PEPB.

Culture medium: RPMI1640 + FCS 10%
Passage method: Dilution.
Growth temperature: 37
Cell No. at passage: 10^5 cells/ml
Additional comments: Viability was 80% prior to freezing. Viability was checked at 07/31/87.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Passage Number, P4*
Date cell culture started:08/21/87
Concentration of cells in an ampule: 6x10^5 cell/ml
Viability under microscope (%): 96%
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, G6PD,LD,NP.

Culture medium: RPMI1640 + FCS 10%
Passage method: Dilution.
Growth temperature: 37
Cell No. at passage: 10^5 cells/ml
Additional comments: Viability was 79 % prior to freezing.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Passage Number, P5*
Date cell culture started:08/25/89
Concentration of cells in an ampule: 3.6x10^6 cell/ml
Viability under microscope (%): 97%
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.

Culture medium: RPMI1640 + FCS 10%
Passage method: Dilution.
Growth temperature: 37
Cell No. at passage: 10^5 cells/ml
Additional comments: Viability was 96.2% before freezing. After frozen viability was checked at 09/20/89.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Passage Number, P9*
Date cell culture started:04/02/90
Concentration of cells in an ampule: 1.5x10^6 cell/ml
Antibiotics used :free
Sterility test has not completed yet.

Culture medium: RPMI1640 + FCS 10%
Passage method: Dilution.
Growth temperature: 37
Cell No. at passage: 10^5 cells/ml
Additional comments: Viability was 84.8% before freezing.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Passage Number, P9*
Cell culture was started with cloning from the previous stock.
Date cell culture started:05/06/90
Concentration of cells in an ampule: 4.4 x 10^6 cell/ml
Viability under microscope (%): 91%
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Anchorage dependency: No

Culture medium: RPMI1640 + FCS 10%
Freezing medium: Culture medium with 5% DMSO.
Passage method: dilution
Growth temperature: 37 C
CO₂ concentration: 5 %
Cell No. at passage: 10^5 cells/ml
Memo: Mycoplasmas tested by staining(Ohnishi) method.
Additional comments: Viability was 81.6% before freezing. Viability 91.2% was checked at 050990.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Passage Number, P7*
Date cell culture started:08/22/90
Concentration of cells in an ampule: 2.3x10^6 cell/ml
Viability under microscope (%): 76%
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.

Culture medium: RPMI1640 + FCS 10%
Passage method: Dilution.
Growth temperature: 37
Cell No. at passage: 10^5 cells/ml
Additional comments: Viability was checkes at 08/22/90.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Cells are propagated from the stock, 021087
Passage Number, P6*
Cell culture was started without cloning from the previous stock.
Date cell culture started:08/30/93
Concentration of cells in an ampule: 4.32x10^6 cell/ml
Saturated cell density: ~1.05x10^6 cells/ml
Growth rate: 28.1hrs.
Viability under microscope (%): 92.9
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, G6PD, LD, PepB.
Anchorage dependency: No
Cell Identification: available

Culture medium: RPMI1640 medium with 10% fetal bovine serum (Hyclone 11151031)
Passage method: Dilution.
Growth temperature: 37 C
Cell No. at passage: 1-2.0x10^5 cells/ml
Memo: from 021087 token. by Okado,K.

[ Additional Data ]

STR-PCR(1), STR-PCR(2),

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(Date downloaded: 2008-06-13)