| Date accepted: | 01/17/1987 |
| Animal: | human |
| Sex and Age: | M :28 year-old |
| Race: | Japanese |
| Scientific Name: | Homo sapiens |
| Case history: | cell clone from the KY821. |
| Tissue prepared: | peripheral blood |
| Medium: | RPMI1640 medium with 10% calf serum |
| Passage method: | Cells are treated with 0.02 % EDTA and 0.25 % trypsin. |
| CO2 Concentration: | 5 % |
| Genetics: | leukemia |
| Characteristics: | The cell was cloned from JCRB0105 by colony formation. |
| Cell ID data: | available |
| DNA Profile (STR): | D5S818:9,10,11,12,13 D13S317:8,9,11,12 D7S820:11,12 D16S539:10,11,12,13 vWA:14,17,18 TH01:7,9 Amelogenin:X TPOX:8,11 CSF1PO:10,12 |
| Established by | Kishi,K. |
| Deposited by | Kishi,K. |
| Cell bank: | NIHS(JCRB) |
| Comments: | Cell ID data indicate some funny results comparing from the KY821(JCRB0105). |
(* The format of the table was revised on Apr.25.2003.)
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No image(s) available. STR-PCR(1), STR-PCR(2), |
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No extradocuments available. |
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[ Lot No.: 032087: token/seed (JCRB0105.1) ] |
Date cell culture started:03/20/87 Concentration of cells in an ampule: 6x10^5 cell/ml Viability under microscope (%): 81% Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, AST,G6PD,LD,MD,MPI,NP,PEPB. Cell Identification: available RFLP data: Pattern of the cell ID has some funny profile, when compared with the KY821(JCRB0105). [ Culture condition of this lot.]
Passage method: 0.02% EDTA and 0.25% trypsin. Growth temperature: 37.0 C Additional comments: Prepared from Dr.KISHI. Viability was 98% prior to freezing. Viability was checked at 09/07/90. [ Additional Data ]
(Date downloaded: 2008-06-13) |
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[ Lot No.: 092990: distribution (JCRB0105.1) ] |
Date cell culture started:09/29/90 Concentration of cells in an ampule: 1.6x10^6 cell/ml Antibiotics used :free [ Culture condition of this lot.]
Passage method: 0.02% EDTA and 0.25% trypsin. Growth temperature: 37 Additional comments: Viability was 94.5% before freezing. [ Additional Data ]
(Date downloaded: 2008-06-13) |
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