JCRB0111:VERO

JCRB0111 [VERO]

Date accepted:	06/05/1987
Other name:	Vero
Animal:	monkey
Strain:	African green
Scientific Name:	Cercopithecus aethiops
Case history:	normal
Classification:	transformed
Tissue prepared:	kidney
Date cell culture started:	06/02/1987
History of the cell:	The cell line was deposited to the JCRB cell bank at passage 111 by Simizu,B., Chiba Univ.
Medium:	Eagle's minimal essential medium with 5% fetal calf serum
Passage method:	Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
CO₂ Concentration:	5 %
Cell no. at passage:	split ratio=1/2 - 1/6
Life span:	infinite
Morphology:	epithelial-like
Characteristics:	Normal cell with contact inhibition
Cell ID data:	available
DNA Profile:	D5S818: D13S317: D7S820:10 D16S539: VWA: TH01: Amelogenin:X TPOX: CSF1PO:14
Established by	Yasumura,Y. & Kawakita
Deposited by	Simizu,B.
Special notice:	No regulation applied.
Cell bank:	NIHS(JCRB)
References:	(1,2,3,4,5,6,7,8)
Comments:	Cells submitted have lower passage number than the cells kept in ATCC.

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Chromosome, Chromosome, Chromosome, Chromosome, Chromosome, Chromosome, STR-PCR(1), STR-PCR(2), Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7),

[ Extra document(s) ]

No extradocuments available.

[ ]

movie1,

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 082185: deposit (JCRB0111) ]

Passage Number,

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

52.0

Tests for mycoplasma, bacteria, and fungi were negative.

[ Culture condition of this lot.]

Culture medium:

Eagle's minimal essential medium with 5% fetal calf serum.

Passage method:

Cells are harvested by the treatment with 0.02 % EDTA and 0.25 % trypsin

Growth temperature:

37 C

Cell No. at passage:

split ratio:1/2 - 1/6

Additional comments:

Prepared at Chiba Univ. Viability was checked at 06/04/87.

[ Additional Data ]

(Date downloaded: 2007-07-11)

[ Lot No.: 061287: token/seed (JCRB0111) ]

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

89.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

AST,GTPD,LD,MD,MPI,NP,PEPB, G6PD, NP..

[ Culture condition of this lot.]

Culture medium:

Eagle's minimal essential medium with 5% fetal calf serum.

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells are harvested after treatment with 0.02 % EDTA and 0.25 % trypsin.

Growth temperature:

37 C

Cell No. at passage:

split ratio=1/2 - 1/6

Additional comments:

Prepared from Simizu, Chiba Univ. Viability was 93% prior to freeze. Viability was checked at 06/09/88.

[ Additional Data ]

Isozyme analysis,

(Date downloaded: 2007-07-11)

[ Lot No.: 061788: distribution (JCRB0111) ]

Passage Number,

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

94%
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

PEPB,NP,LD.

[ Culture condition of this lot.]

Culture medium:

MEM + FCS 5 %

Passage method:

0.02 % EDTA and 0.25 % trypsin

Growth temperature:

Cell No. at passage:

split ratio:1/2 - 1/6

Additional comments:

Viability was 98.9% prior to freeze. Viability 94% was checked at 01/18/89.

[ Additional Data ]

(Date downloaded: 2007-07-11)

[ Lot No.: 092990: distribution (JCRB0111) ]

Passage Number,

Date cell culture started:

Concentration of cells in an ampule:

Antibiotics used :

Tested Isoenzymes

G6PD, LD, NP.

[ Culture condition of this lot.]

Culture medium:

MEM + FCS 5 %

Passage method:

0.02 % EDTA and 0.25 % trypsin

Growth temperature:

Cell No. at passage:

split ratio:1/2 - 1/6

Additional comments:

Viability was 97.7% before freezing.

[ Additional Data ]

(Date downloaded: 2007-07-11)

[ Lot No.: 072793: distribution (JCRB0111) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

87.2
Antibiotics used :free
Virus detected :+
Anchorage dependency:

Yes

[ Culture condition of this lot.]

Culture medium:

Eagle's minimal essential medium with 5% fetal bovine serum (J.R.H.Biosciences 1B1062)

Passage method:

Cells are treated with 0.25% trypsin and 0.02% EDTA.

Growth temperature:

37 C

Cell No. at passage:

1-2x10^5 cells/ml

Memo:

[ Additional Data ]

(Date downloaded: 2007-07-11)

[ Lot No.: 072393: for BVD/Myco test (JCRB0111) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Antibiotics used :

Sterility test has not completed yet.

Virus detected :

Anchorage dependency:

Yes

Cell Identification:

available

[ Culture condition of this lot.]

Culture medium:

Eagle's minimal essential medium with 5% fetal bovine serum (JRH Biosciences, 1B1062)

Passage method:

Harvest cells after treatment with 0.25% trypsin and 0.02% EDTA. Cell aggregates removed w 150 mesh.

Growth temperature:

37 C

Cell No. at passage:

1-2x10^4 cells/ml

Memo:

[ Additional Data ]

Mycoplasma (PCR),

Chromosome,

STR-PCR(1),

STR-PCR(2),

(Date downloaded: 2007-07-11)

[ Lot No.: 01172003: test (JCRB0111) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

98.2
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD, NP, LDH examined. Monkey confirmed..

Anchorage dependency:

Yes

Cell Identification:

available

[ Culture condition of this lot.]

Culture medium:

Eagle's minimal essential medium with 5% fetal calf serum (Intergen RB52305).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells were harvested after treatment with 0.25% trypsin.

Growth temperature:

37 C

CO₂ concentration:

5 %

Cell No. at passage:

3.3 x 10^4 cells/sq.cm.

[ Additional Data ]

Cells(1),

Cells(2),

Cells(3),

Mycoplasma test,

Mycoplasma (PCR),

STR-PCR(1),

STR-PCR(2),

Freezing process,

(Date downloaded: 2007-07-11)

[ Lot No.: 021390: research (JCRB0111) ]

Passage Number,

[ Culture condition of this lot.]

Memo:

Additional comments:

Culture sheet was not found.

[ Additional Data ]

(Date downloaded: 2007-07-11)

[ Lot No.: 12012006: test (JCRB0111) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

97.1
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

Yes

[ Culture condition of this lot.]

Culture medium:

Eagle's minimal essential medium with 5% heat-inactivated fetal bovine serum

Freezing medium:

Culture medium with 5% DMSO

Passage method:

Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.

Growth temperature:

37 C

CO₂ concentration:

5 %

Cell No. at passage:

3.3x10^3 cells/sq.cm

[ Additional Data ]

Cells(1),

Cells(2),

Cells(3),

Mycoplasma test,

Mycoplasma (PCR),

Freezing process,

(Date downloaded: 2007-07-11)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Yasumura,Y. and Kawakita,M. The research for the SV40 by means of tissu culture technique, Nippon Rinsho, 21(6): 1201-1219, 1963.

2. Sasaki,K., Makino,S., and Kasahara,S. Studies on measles virus, II. Propagation in two established simian renal cell lines and development of a plaque assay, Kitasato Arch. Exp. Med., 37: 27-42, 1964.

3. Earley,E., Peralta,P.H., and Johnson,K.M. A plaque neutralization method for arboviruses., Proc. Soc. Exp. Biol. Med., 125: 741, 1967.

4. Liebhaber,H., Riordan,J.T., and Horstmann,D.M. Replication of rubella virus in a continuous line of African green monkey kidney cells (Vero), Proc. Soc. Exp. Biol. Med., 125: 636-643, 1967.

5. Simizu,B., Rhim,J.S., and Wiebenga,N.H. Characterization of the Tacaribe group of arboviruses. Propagation and plaque assay of Tacaribe virus in a line of African green monkey kedney cells (Vero) (32029), Proc. Soc. Exp. Biol. Med., 124: 119-123, 1967.

6. Rhim,J.S., Simizu,B., and Wiebenga,N.H. Growth of Junin virus, the etiological agent of Argentinian hemorrhagic fever, in cell cultures, Arch. Gesamte. Virusforsch., 21: 243-252, 1967.

7. Sasaki,K., Makino,S., and Kasahara,S. Studies on measles virus. II. Propagation in two established simian renal cell lines and development of a plaque assay, Kitasato Arch. Exp. Med., 37: 27-42, 1964.

8. Youil,R., Su,Q., Toner,T.J., Szymkowiak,C., Kwan,W.S., Rubin,B., Petrukhin,L., Kiseleva,I., Shaw,A.R., and DiStefano,D. Comparative study of influenza virus replication in Vero and MDCK cell lines, J. Virol. Methods, 120: 23-31, 2004.