JCRB0112.1:THP-1

JCRB0112.1 [THP-1]

Profile:Human acute monocytic leukemia.

Date accepted: 08/05/1987

Other name: THP-1/ATCC

Animal: human

Sex and Age: M :young

Race: Japanese

Scientific Name: Homo sapiens

Case history: acute monocytic leukemia

Classification: tumor

Tissue prepared: peripheral blood

Origin of tumor: peripheral blood

Date cell culture started: 08/04/87

History of the cell: Tsuchiya,S.=>NIHS(JCRB)

Medium: RPMI1640 medium with 5% fetal calf serum.

Passage method: Dilution, twice a week.

CO₂ Concentration: 5 %

Cell no. at passage: 2-5x10^5 cells/ml

Genetics: Fc,C3b receptor.HLA-A2,-A9,-B5,-DRW1,-DRW2.

Life span: infinite

Morphology: lymphocyte-like

Characteristics: No immuno glogulin productions.

Cell ID data: available

DNA Profile (STR): D5S818:11,12
D13S317:13
D7S820:10
D16S539:11,12
VWA:16
TH01:8,9.3
Amelogenin:X,Y
TPOX:8,11
CSF1PO:11,13

Virus DNA detected: CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested] -- Description

Established by Tsuchiya,S.

Deposited by Tsuchiya,S.

Special notice: Regulation not applied.

Cell bank: NIHS(JCRB)

References: (1),(2),(3),(4),(5),(6),(7)

Comments: HLA-DR, My7, My9, OKM1 positive. Nonspecific peroxidase positive, peroxidase negative, a-naftol ASD chloroacetate esterase negative. by Dr. Tsuchiya.

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Mycoplasma test, Isozyme analysis, STR-PCR(1), STR-PCR(2), STR-PCR(3), Freezing process,

[ Extra document(s) ]

Additional Information(1)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 082687: token/seed (JCRB0112.1) ]

Passage Number,

Cell culture was started with cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

90%
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

AST,G6PD,LD,MD,MPI,NP,PEPB.

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

RPMI1640 + FCS 5%

Passage method:

Dilution, twice a week

Growth temperature:

Cell No. at passage:

2-5x10^5 cells/ml

Additional comments:

Viability was 98% prior to freeze. Viability=90% was checked at 11/10/88.

[ Additional Data ]

STR-PCR(1),

STR-PCR(2),

STR-PCR(3),

(Date downloaded: 2011-02-17)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Tsuchiya,S., Yamabe,M., Yamaguchi,Y., Kobayashi,Y., Konno,T., and Tada,K. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1), Int. J. Cancer, 26: 171-176, 1980.

2. Tsuchiya,S., Kobayashi,Y., Goto,Y., Okumura,H., Nakae,S., Konno,T., and Tada,K. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester, Cancer Res., 42: 1530-1536, 1982.

3. Palumbo,A., Minowada,J., Erikson,J., Croce,C.M., and Rovera,G. Lineage infidelity of a human myelogenous leukemia cell line., Blood, 64: 1059-1063, 1984.

4. Hemmi,H. and Breitman,T.R. Induction of functional differentiation of a human monocytic leukemia cell line (THP-1) by retinoic acid and cholera toxin, Jpn. J. Cancer Res., 76: 345-351, 1985.

5. Tajima,S., Hayashi,R., Tsuchiya,S., Miyake,Y., and Yamamoto,A. Cells of a human monocytic leukemia cell line (THP-1) synthesize and secrete apolipoprotein E and lipoprotein lipase, Biochem. Biophys. Res. Commun., 126: 526-531, 1985.

6. Hemmi,H., Nakamura,T., Tamura,T., Shimizu,Y., Kato,S., Miki,T., Takahashi,N., Muramatsu,M., Numao,M., and Sugamura,K. Lymphotoxin; induction of terminal differentiation of the human myeloid leukemia cell lines HL-60 and THP-1, J. Immunol., 138: 664-666, 1987.

7. Mayumi,M., Kawabe,T., Kim,K.-M., Heike,T., Katamura,K., Yodoi,J., and Mikawa,H. Regulation of Fce receptor expression on a human monoblast cell line U937., Clin. Exp. Immunol., 71: 202-206, 1988.