Passage Number, P6*
Cell culture was started without cloning from the previous stock.
Date cell culture started:05/21/91
Concentration of cells in an ampule: 3.0x10^6 cell/ml
Viability under microscope (%): 97.5
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Cell Identification: available

Culture medium: RPMI1640 medium with 10% fetal calf serum
Freezing medium: Culture medium with 5% DMSO
Passage method: dilution
Growth temperature: 37 C
CO₂ concentration: 5%

[ Additional Data ]

STR-PCR(1), STR-PCR(2),

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(Date downloaded: 2008-06-13)

Passage Number, P6*
Cell culture was started without cloning from the previous stock.
Date cell culture started:05/21/91
Concentration of cells in an ampule: 3.0x10^6 cell/ml
Viability under microscope (%): 97.5
Antibiotics used :free

Culture medium: RPMI1640 + FCS 10%
Growth temperature: 37 C
Additional comments: Viability was 97.5% before freezing.

[ Additional Data ]

(Date downloaded: 2008-06-13)

Passage Number, P10*
Cell culture was started without cloning from the previous stock.
Date cell culture started:01/07/94
Concentration of cells in an ampule: 3.7x10^6 cell/ml
Viability under microscope (%): 81.2
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, LD,PepB.
Anchorage dependency: No

Culture medium: RPMI-1640 medium with 10% heat inactivated fetal vobine serum (JRS.DO10400)
Passage method: Dilution.
Growth temperature: 37 C
Cell No. at passage: 2-3x10^5 cells/ml
Memo: By Ohnishi,K.

[ Additional Data ]

(Date downloaded: 2008-06-13)