JCRB0126.1:KOSC-2 cl3-43

JCRB0126.1 [KOSC-2 cl3-43]

Profile:Human tumor cells derived from oral cancer at stage III. The cells were originally cross contaminated with mouse cells but the mouse cells were eliminated in the JCRB cell bank and the cl3-43 subclone ID was applied to the cell name.

Date accepted: 09/10/1998

Other name: KOSC-2

Previous cell Number: JCRB0126

Animal: human

Sex and Age: M :51-year old

Scientific Name: Homo sapiens

Case history: oral cancer at stage III. Mouse cells eliminated by cell cloning & mycoplasmas eliminated by MC210.

Classification: tumor

Tissue prepared: oral floor

Origin of tumor: oral floor

Date cell culture started: 05/26/98

History of the cell: Contaminated mouse cells were eliminated by limiting dilution in the JCRB Cell Bank after deposited.

Medium: RPMI-1640 medium with 10% fetal bovine serum.

Passage method: Cells are passaged twice a week by trypsinization.

CO₂ Concentration: 5 %

Cell no. at passage: 2.2 x 10^4 cells/ml

Life span: infinite

Morphology: epithelial-like

Characteristics: Moderately differentiated squamous-cell carcinoma.

Cell ID data: available

DNA Profile (STR): D5S818:9
D13S317:11
D7S820:10,11
D16S539:9,12
vWA:15,17
TH01:6,7
Amelogenin:X
TPOX:8
CSF1PO:12

Virus DNA detected: CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested] -- Description

Established by Inagaki,T.

Deposited by Inagaki,T.

Special notice: free

Cell bank: NIHS(JCRB)

References: (1)

Comments: Cells were established through xenografted tumors in nude mice by Geneticin treatment to eliminate mouse fibroblast cells. Mouse cells observed when deposited.

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Chromosome, STR-PCR(1), STR-PCR(2), Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

Additional Information(1)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 061998: seed (JCRB0126.1) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Growth rate:

Viability under microscope (%):

97.1
Antibiotics used :free [PDT=(t'-t)xlog2/(logN'-logN)]

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(type B), NP and LDH examined. human non-HeLa..

Kariology:

Chromosome frequency distribution:

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

RPMI1640 medium with 10% or 15% fetal bovine serum(Mitsubishi PVF01).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells harvested with 0.25% trypsin and 0.02% EDTA at 37 C for 5-10 min and adjusted cell number.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

2.0 x 10^4 cells/sq.cm.

Memo:

[ Additional Data ]

Mycoplasma test,

Isozyme analysis,

Chromosome,

Freezing process,

(Date downloaded: 2008-06-13)

[ Lot No.: 030899: seed (JCRB0126.1) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

92.7
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(type B), LD and NP. Human. Not HeLa..

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

RPMI1640 medium with 10% fetal bovine serum (Biocell,L.4002171).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells harvested with 0.25% trypsin and 0.02% EDTA when subculture.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

1.2 x 10^4 cells/sq.cm.

Memo:

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

Freezing process,

(Date downloaded: 2008-06-13)

[ Lot No.: 08222000: distribution (JCRB0126.1) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

93.4
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(typB), NP, LDH examined by Sasaki,A. (human).

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

RPMI1640 medium with 10% fetal bovine serum (Mitsubishi PVF01).

Freezing medium:

Culture medium containing 5% DMSO.

Passage method:

Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temerature for 5 minutes. Change medium every 2-3 days and subculture every 5 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

7.0 x 10^3 cells/sq.cm.

Additional comments:

Cells prepared by Kurematsu,M. Mycoplasmas tested by staining method and PCR(Takada,Y.).

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

STR-PCR(1),

STR-PCR(2),

Freezing process,

(Date downloaded: 2008-06-13)

[ Lot No.: 08102000: distribution (JCRB0126.1) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

94.6
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(Type B), LDH, NP examined. Human..

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

RPMI1640 medium with 10% fetal bovine serum (Mitsubishi PVF01).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Change medium every 2-3 days and subculture every 5 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

7.0 x 10^3 cells/sq.cm.

Additional comments:

Cells prepared by Kurematsu,M. Mycoplasma tested by both staining and PCR.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

STR-PCR(1),

STR-PCR(2),

Freezing process,

(Date downloaded: 2008-06-13)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Inagaki,T., Matsuwari,S., Takahashi,R., Shimada,K., Fujie,K., and Maeda,S. Establishment of human oral-cancer cell lines (KOSC-2 and -3) carrying p53 and C-myc abnormalities by geneticin treatment, Int. J. Cancer, 56: 301-308, 1994.