JCRB0128:TK-1

JCRB0128 [TK-1]

Profile:Human glioblastoma multiforme cell line. Cross-Culture contamination with U251 suspected.

Date accepted: 03/20/1998

Previous cell Number: NIHS0181

Animal: human

Sex and Age: F :22-years-old

Scientific Name: Homo sapiens

Case history: glioblastoma multiforme

Classification: tumor

Tissue prepared: brain

Origin of tumor: brain

Date cell culture started: 06/08/94

History of the cell: Originally established by the depositor's laboratory.

CO₂ Concentration: 5 %

Life span: infinite

Crisis PDL: NT

Morphology: fibroblast-like

Cell ID data: available

DNA Profile (STR): D5S818:11,12
D13S317:10,11
D7S820:10,12
D16S539:12
vWA:16,18
TH01:10
Amelogenin:XY
TPOX:8
CSF1PO:11,12

Virus DNA detected: CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested] -- Description

Established by Tsuboi,K.

Deposited by Tsuboi,K.

Cell bank: NIHS(JCRB)

References: (1)

Comments: STR-PCR experiments suggested the cross-culture contamination with the U251 (RCB0461).

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, STR-PCR(1), STR-PCR(2), STR-PCR(3), Growth Curve, Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

Additional Information(1)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 041798: seed (JCRB0128) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

99.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

Cell Identification:

RFLP data:

[ Culture condition of this lot.]

Culture medium:

Eagle's minimal essential medium with 10% fetal calf serum (Mitsubishi, PVF01).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells are harvested with 0.25% trypsin and 0.5% EDTA for 5min.at room temp. Subculture every 6 days.

Growth temperature:

Cell No. at passage:

1-2 x 10^3 cells/sq.cm.

Memo:

Additional comments:

The U251 is available from RIKEN cell bank (RCB0461).

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

STR-PCR(1),

STR-PCR(2),

STR-PCR(3),

(Date downloaded: 2008-06-13)

[ Lot No.: 063098: distribution (JCRB0128) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

89.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(typeB), LD, NP. Human..

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

Eagle's minimal essential medium with 10% fetal bovine serum (lot.Rehatuin LB94203).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Subculture every 4-5 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

1.6 x 10^4 cells/sq.cm.

Memo:

[ Additional Data ]

Mycoplasma test,

Isozyme analysis,

Freezing process,

(Date downloaded: 2008-06-13)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Tsuboi,K., Yoshii,Y., and Nose,T. Establishment of human glioblastoma cell line TK-1, Hum. Cell, 9: 125-128, 1996.