Cells are propagated from the stock, deposit
Passage Number, P4*
Cell culture was started without cloning from the previous stock.
Date cell culture started:07/16/2004
Concentration of cells in an ampule: 4.3 x 10^6 cell/ml
Viability under microscope (%): 96.2
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, G6PD, NP, LDH examined. Rat..
Anchorage dependency: Yes

Culture medium: DM201 medium. Serum free cell culture.
Freezing medium: 1 to 1 mixture of the culture medium and FM-1 medium containing 10% DMSO.
Passage method: Cells were harvested with scraper. Subculture every 4-7 days and medium change every 2-3 days.
Growth temperature: 37 C
CO₂ concentration: 5 %
Cell No. at passage: 2.0 x 10^4 cells/sq.cm.
Additional comments: Mycoplasmas tested by PCR(Ozawa,Y.) and staining method.

[ Additional Data ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Freezing process,

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(Date downloaded: 2008-06-13)