JCRB0132.3:AH601.P3(JTC27)

JCRB0132.3 [AH601.P3(JTC27)]

Profile:AH-601 cell line adupted to a serum free medium with long passage history.

Date accepted: 06/09/2004

Other name: JTC27.P3

Previous cell Number: NIHS0357

Animal: rat

Scientific Name: Rattus norvegicus

Case history: tumor transplantable, Hepatoma induced by feeding aminoazotoluene.

Classification: tumor

Histological type of the cell: hepatoma

Tissue prepared: acites

Origin of tumor: hepatoma

Date cell culture started: --/--/1971

History of the cell: Katsuta,H. (Takaoka,T.) -> JCRB cellbank, Low level mycoplasma detected when deposited.

Medium: DM201 medium. No serum.

Passage method: Cells are harvested with scraper.

CO₂ Concentration: 5 %

Cell no. at passage: 5x10^4 cells/sq.cm.

Genetics: AH-601 cells adupted to a serum free medium.

Life span: infinite

Morphology: epithelial-like

Characteristics: transplantable rat ascites tumor P3 cell line and cells have been cultured continuously for long period in the serum free medium. Teromerase positive and a length of telomere is 5.1 kb. Td=50 hrs.

Established by Huh,N.,Takaoka,T. & Katsuta,H.

Deposited by Takaoka,T.

Special notice: free

Cell bank: NIHS(JCRB)

References: (1)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

Additional Information(1)
Additional Information(2)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 06302004: seed (JCRB0132.3) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

96.2
Antibiotics used :MC210 used to remove mycoplasmas

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

DM201 with MC210(for this lot only).

Freezing medium:

Culture medium containing 5% DMSO.

Passage method:

Cells were harvested after treatment with pipetting or scraper. Subculture every 4-6 days and medium change every 3-4 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

5-9 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasmas decontaminated on this lot by MC210.

[ Additional Data ]

Mycoplasma test,

Freezing process,

(Date downloaded: 2008-06-13)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Huh,N., Takaoka,T., and Katsuta,H. Tissue culture of rat ascites hepatoma AH-601 cells, Jpn. J. Exp. Med., 42: 249-262, 1972.