JCRB0144.1D:LYM-1.P3D

JCRB0144.1D [LYM-1.P3D]

Profile:Serum free FAR-2.F60 rat cell culture from lymph node near colon cancer.

Date accepted: 06/09/2004

Previous cell Number: NIHS0360

Registerd cell number: JCRB0144.1D

Animal: rat

Strain: JAR-2

Scientific Name: Rattus norvegicus

Case history: tissue obtained from lymph node near colon cancer of the JAR-2 rat given MNU(0.4mg/ml). The MNU was applied to a bowel directly of two-week-old rat and tumors were found three mo. later. The lymph node was cultured by roller tubes with DM170 and 2% FBS.

Classification: serum free

Tissue prepared: lymph node near colon cancer

Date cell culture started: 10/04/1982

History of the cell: Cells established by the original developper and converted to a serum free cell culture. The cells were then serially subcultured for long time and finally the cell culture was deposited to the JCRB cell bank at 2004.

Medium: DM201 medium with serum free condition.

Passage method: Cells harvested by gentle scraping.

Cell no. at passage: 1.3 x 10^4 cells/sq.cm.

Genetics: Subclone of the LYM-1.P3. Morphologys and karyotypes were different from the LYM-1.P3B. Karyotype is lower diploid.

Life span: infinite

Morphology: fibroblast-like

Characteristics: Culture fruid of the LYM-1 strongly inhibited a growth of AH-7974 and other several cell lines. The inhibition was strongly suppressed by heating.

Established by Kurayama,H. and Takaoka,T.

Deposited by Takaoka,T.

Special notice: free

Cell bank: NIHS(JCRB)

References: (1,2)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

Additional Information(1)
Additional Information(2)
Additional Information(3)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 07152004: distribution (JCRB0144.1D) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

91.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

LDH,NP,G6PD examined. Rat proved..

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

DM201 serum free medium.

Freezing medium:

1 to 1 mixture of the culture medium and the FM-1 medium containing 10% DMSO.

Passage method:

Cells were harvested after treatment with scraper.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

2.0 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasma test done by PCR methods(Takada,Y.).

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

Freezing process,

(Date downloaded: 2008-06-13)

[ Lot No.: 07142004: seed (JCRB0144.1D) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

91.1
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD, NP, LDH examined. Rat confirmed..

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

DM201, serum free medium.

Freezing medium:

1 to 1 mixture of the culture medium and the FM-1 medium, containing 10% DMSO.

Passage method:

Cells were harvested with scraper. Subculture every 4-6 days, and medium change every 3-4 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

2.0 x 10^4 cells/sq.cm.

[ Additional Data ]

Mycoplasma test,

Isozyme analysis,

Freezing process,

(Date downloaded: 2008-06-13)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Kurayama,H., Ogata,S., Nanba,M., Ikeguchi,S., Takaoka,T., and Shida,S. Establishment of a new cell line secreting colony stimulating factor and growth inhibiting factor from metastatic lymphnodes of a rat, Dokkyo J. Med. Sci., 13: 9-13, 1986.

2. Wang,X.M., Kojima,T., Satoh,K., Taniguchi,Y., Tokumaru,K., Saifuku,K., Seki,M., Kihira,K., Ido,K., Uchida,J.Y., Ohmori,C., Takaoka,T., and Kimura,K. The value of LYM-1 cells for examining vacuole formation and loss of cell viability induced by culture supernates of Helicobacter pylori, J. Med. Microbiol., 46: 705-709, 1997.