JCRB0157:TK

JCRB0157 [TK]

Profile:Human B-cell lymphoma cell line with near-triploid chromosomes with t(14;18) and p53 gene point mutation.

Date accepted: 10/27/2000

Previous cell Number: NIHS0245

Animal: human

Sex and Age: F :80-year-old

Scientific Name: Homo sapiens

Case history: B-cell malignant lymphoma.

Classification: tumor

Tissue prepared: peripheral blood

Origin of tumor: peripheral blood

Date cell culture started: 10/14/1996

History of the cell: Cells deposited from the original developer.

Medium: RPMI1640 medium with 10% fetal calf serum

Passage method: dilution

CO₂ Concentration: 5 %

Genetics: CD10, CD19, CD20, HLA-DR positive. A codon 180 of the p53 mutated (GAG -> GAT). Near-triploid chromosomes with t(14;18)(q32;q21), add(17)(p22).

Life span: infinite

Morphology: lymphocyte-like

Characteristics: A rare large B-cell non-Hodgkin's lymphoma having a near-triploid cell population.

Established by Inokuchi,K. et.al.

Deposited by Inokuchi,K.

Special notice: not defined

Cell bank: NIHS(JCRB)

References: (1)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

No extradocuments available.

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 01292001: seed (JCRB0157) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

92.8
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

NP, G6PD, LDH examined. (human G6PD type B)..

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

alpha-MEM with 10% heat inactivated fetal calf serum (Intergen RB2405).

Freezing medium:

Culture medium containing 5% DMSO.

Passage method:

Cells harvested by simple dilution. Suspension cell culture.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

1.0 x 10^5 cells/sq.cm.

Additional comments:

Cells propagated by Kurematsu,M. Mycoplasmas examined by PCR.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

Freezing process,

(Date downloaded: 2008-06-13)

[ Lot No.: 01302001: distribution (JCRB0157) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

92.8
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

alpha-MEM with 10% heat inactivated fetal calf serum (Intergen RB2405).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Subculture was done by simple dilution.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

Additional comments:

Cells propagated by Kurematsu,M. Mycoplasmas tested by PCR.

[ Additional Data ]

Mycoplasma test,

Freezing process,

(Date downloaded: 2008-06-13)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Yamaguchi,H., Inokuchi,K., Hanawa,H., Tanosaki,S., Shinozawa,I., Matuoka,H., and Dan,K. Establishment of a near-triploid human B-cell lymphoma cell line with t(14;18) and p53 gene point mutation, Br. J. Haematol., 105: 764-767, 1999.