JCRB0173 [SKN]

Date accepted:	02/08/1990
Previous cell Number:	FDSC0031
Animal:	human
Sex and Age:	F :52-year-old
Race:	Indonesian
Scientific Name:	Homo sapiens
Case history:	uterus leiomyoma
Classification:	tumor
Tissue prepared:	uterus
Origin of tumor:	uterus
History of the cell:	Ishiwatari,I. -> FDSC -> JCRB
Medium:	Ham's F12 medium 90% with 10% fetal bovine serum.
Passage method:	Cells are harvested after treated with 0.02% EDTA and 0.1% trypsin. 37 C.
CO2 Concentration:	5 %
Cell no. at passage:	5.7 x 10^4 cells/sq.cm.
Genetics:	Mode of chromosome = 104-112.
Life span:	infinite
Characteristics:	Histochemical characteristics = PP2, PP6, PP7
Cell ID data:	available
DNA Profile (STR):	D5S818:12 D13S317:11 D7S820:8,12 D16S539:9 VWA:19 TH01:8 Amelogenin:X TPOX:8 CSF1PO:10,11
Virus DNA detected:	CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested] -- Description
Established by	Ishiwatari,I. & Nozawa,S.
Deposited by	Nozawa,S.
Cell bank:	NIHS(JCRB)
References:	(1)
Comments:	Cell culture started by explant culture method. Doubling time = 36 hrs. Rate of colony formation = 21.3%. Passage number is over 117 when deposited. Unique by STR-PCR(10/17/2001).

(* The format of the table was revised on Apr.25.2003.)

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Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, STR-PCR(1), STR-PCR(2), Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7),





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(Date downloaded: 2011-10-17)

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