Cells are propagated from the stock, deposit
Passage Number, P30
Cell culture was started without cloning from the previous stock.
Date cell culture started:07/30/96
Concentration of cells in an ampule: 3.95x10^6 cell/ml
Culture medium: Dulbecco's modified Eagle's medium with 10% fetal bovine serum (JRH Biosciences,lot.1B1062).
Freezing medium: 5% DMSO with culture medium.
Passage method: Cells washed by PBS(-) once and treated with 0.25%trypsin-0.02% EDTA.
Growth temperature: 37 C
Cell No. at passage: 2-4x10^5 cells/sq.cm
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Anchorage dependency: Yes
Memo: From lot.deposit by Minegishi,D.

[ Additional Data ]

Cells(1), Cells(2), Mycoplasma test, Mycoplasma (PCR), Freezing process,

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(Date downloaded: 2003-03-26)

Passage Number, P30
Cell culture was started without cloning from the previous stock.
Date cell culture started:07/30/96
Concentration of cells in an ampule: 3.95x10^6 cell/ml
Culture medium: Dulbecco's modified Eagle's medium with 10% fetal Bovine serum (JRH Biosciences lot.1B1062).
Freezing medium: 5% DMSO with culture medium.
Passage method: Cells washed once with PBS(-) once and treated with 0.25% trypsin and 0.02% EDTA.
Growth temperature: 37 C
Cell No. at passage: 2-4x10^5 cells/sq.cm.
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, G6PD, NP, LDH examined. Rat confirmed..
Anchorage dependency: Yes

[ Additional Data ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Freezing process, Others(1), Others(2),

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(Date downloaded: 2003-03-26)