JCRB0262:T.T

JCRB0262 [T.T]

Profile:Human squamous cell carcinoma derived from esophageal cancer.

Date accepted: 07/08/1989

Animal: human

Sex and Age: M :67-year-old

Scientific Name: Homo sapiens

Case history: esophageal cancer operated. 30Gy irrad. 50mgBMLed.

Classification: tumor

Tissue prepared: esophagus

Origin of tumor: esophagus

Metastatic tissue: oral cavity

Date cell culture started: 11/01/83

Medium: Dulbecco's modified Eagle's medium with 45% Ham's F12 medium and 10% fetal calf serum

Passage method: Cells are treated with 0.02% EDTA and 0.1% trypsin.

CO₂ Concentration: 5 %

Cell no. at passage: 10^5 cells/60mm dish

Genetics: squamous carcinoma

Life span: infinite

Morphology: epithelial-like

Characteristics: 3p-, 6q- Mode=85-87(chr)

Cell ID data: available

DNA Profile (STR): D5S818:8,10
D13S317:11
D7S820:11
D16S539:9,11
vWA:17
TH01:9
Amelogenin:XY
TPOX:8,11
CSF1PO:11,13

Virus DNA detected: CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested] -- Description

Established by Takahashi,K. et al.

Deposited by Takahashi, K.

Cell bank: NIHS(JCRB)

References: (1,2,3)

Comments: Derived from mandibular metastasis.

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Chromosome, Chromosome, Chromosome, STR-PCR(1), STR-PCR(2), Freezing process,

[ Extra document(s) ]

Additional Information(1)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 070889: token/seed (JCRB0262) ]

Passage Number,

Date cell culture started:

Concentration of cells in an ampule:

Antibiotics used :

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

LD,G6PD,NP,AST,PepB,MD,MPI.

[ Culture condition of this lot.]

Culture medium:

DMEM + Ham's F12 (1:1) + FCS 10%

Passage method:

0.02% EDTA and 0.1% trypsin.

Growth temperature:

Cell No. at passage:

Additional comments:

Viability was 95% prior to freezing.

[ Additional Data ]

(Date downloaded: 2008-07-09)

[ Lot No.: 071195: FDSC/distribution (JCRB0262) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

99.0
Antibiotics used :free

Tests for bacteria and fungi were netative, but mycoplasma test has not completed.

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium with Ham's F12(1:1 mix) mixed medium with 10% fetal bovine serum.

Passage method:

Treat cells with 0.25% trypsin and 0.02% EDTA.

Growth temperature:

Cell No. at passage:

9.8x10^4 cells/ml

Memo:

[ Additional Data ]

(Date downloaded: 2008-07-09)

[ Lot No.: 021492: transfer (JCRB0262) ]

Cells are propagated from the stock,

Passage Number,

Cell Identification:

[ Culture condition of this lot.]

Memo:

[ Additional Data ]

Mycoplasma (PCR),

STR-PCR(1),

STR-PCR(2),

(Date downloaded: 2008-07-09)

[ Lot No.: 02142001: (JCRB0262) ]

Tests for mycoplasma, bacteria, and fungi were negative.

[ Culture condition of this lot.]

CO₂ concentration:

Additional comments:

Mycoplasma tested by PCR(Takada,Y.).

[ Additional Data ]

(Date downloaded: 2008-07-09)

[ Lot No.: 12202001: seed (JCRB0262) ]

Cells are propagated from the stock,

Passage Number,

Date cell culture started:

Tested Isoenzymes

G6PD(TypeB), NP, LDH examined. Human not a HeLa..

[ Culture condition of this lot.]

[ Additional Data ]

Isozyme analysis,

Chromosome,

Chromosome,

Chromosome,

(Date downloaded: 2008-07-09)

[ Lot No.: 12212001: distribution (JCRB0262) ]

Passage Number,

[ Culture condition of this lot.]

[ Additional Data ]

(Date downloaded: 2008-07-09)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Takahashi,K., Kanazawa,H., Hosono,R., Takahara,M., Mutoh,T., and Sato,K. [Hito Shokudo Henpei Johi-gan no Kagaku-kotsu teni-so yori erareta Saibo-Kabu no Juritsu to sono Seijo], Abstruct for the Ann. Meeting of the Jpn. Can. Assoc., 47: 289(No.1018), 1988.

2. Yokoe,H., Takahashi,K., Kanazawa,H., and Sato,K. Establishment of two cell lines derived from oral metastasis of a human esophageal carcinoma, Abstruct, Annual meeting for the Human Cell Assoc. Jpn., 7: 1988.

3. Takahashi,K., Kanazawa,H., Chan,C.-T., Hosono,T., Takahara,M., and Sato,K. A case of esophageal carcinoma metastatic to the mandible and characterization of two cell lines (T.T. and T.Tn) established from the oral tumor, Jpn. J. Oral. Maxillofac. Surg., 36: 307-316, 1990.