| Profile:Human normal diploid fibroblast cell line transformed with SV40(ts) early gene. Cell culture at 34 C. |
| Date accepted: | 03/08/2002 |
| Previous cell Number: | new |
| Animal: | human |
| Sex and Age: | M Ffetal |
| Race: | Japanese |
| Scientific Name: | Homo sapiens |
| Tissue: | lung |
| Medium: | Eagle's minimal essential medium with 10% fetal bovine serum. |
| Passage method: | Cells are harvested after treatment with 0.25% trypsin. |
| Cell no. at passage: | split ratio = 1/4. |
| Case history: | SV40 transformed TIG-3 cell. |
| Genetics: | TIG-3 cells(JCRB0506) containing SV40 early gene with ts mutation. |
| Life span: | infinite |
| Morphology: | fibroblast-like |
| Characteristics: | Human normal fibroblast with immortal life span by the introduction of the SV40 early gene. |
| Classification: | transformed |
| Established by | Ide,T. |
| Deposited by | TIG |
| Cell bank maintaining these cells. | TIG(JCRB) |
| Comments: | Culture at 34 C becuase of the ts cell line. |
| Cells(1), |
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No extradocuments available. |
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[ Lot No.: 03082002: seed (JCRB0506.1) ] |
Cell culture was started without cloning from the previous stock. Culture medium: Eagle's minimal essential medium with 10% fetal bovine serum. Freezing medium: Culture medium with 15 % DMSO. Passage method: Cells harvested after the treatment with 0.25% trypsin. Growth temperature: 34 C (ts) CO2 concentration: 5 % Cell No. at passage: split 1/4 Sterility test has not completed yet. Anchorage dependency: Yes [ Additional Data ]
(Date downloaded: 2002-04-25) |
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[ Lot No.: 03252002: seed/distribution (JCRB0506.1) ] |
Cell culture was started without cloning from the previous stock. Culture medium: Eagle's minimal essential medium with 10% fetal bovine serum. Freezing medium: Culture medium with 15 % DMSO. Passage method: Cells harvested after the treatment with 0.25% trypsin. Growth temperature: 34 C (ts) CO2 concentration: 5 % Cell No. at passage: split 1/4 Sterility test has not completed yet. Anchorage dependency: Yes Comments: This lot was prepared as a first distribution stock in HSRB but stored as a seed stock in the JCRB. [ Additional Data ]
(Date downloaded: 2002-04-25) |
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