| Date accepted: | 03/02/85 |
| Other name: | RLG-1 |
| Animal: | rat, JAR-1 |
| Scientific Name: | Rattus norvegicus |
| Tissue: | lung |
| Medium: | DM160 medium with 10% fetal bovine serum |
| Passage method: | Rubber policeman or trypsin |
| Genetics: | lung |
| Characteristics: | lung |
| Established by | Katsuta,H. |
| Deposited by | Kuroki,T. |
| Cell bank maintaining these cells. | TIMS(JCRB) |
| References: | (1) |
| Cell culture, Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Freezing process, |
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No extradocument. |
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[ Lot No.:093097: seed (JCRB0608) ] |
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Date cell culture started:09/19/97 Concentration of cells in an ampule: 1.47x10^6 cell/ml Culture medium: DM160 medium with 10% fetal bovine serum (JRH Biosciences, lot.1B1062). Growth temperature: 37 C Freezing medium: Culture medium with 5% DMSO. Passage method: Cells are treated with trypsin when subculture the cells. Cell No. at passage: 1.6x10^4 cells/sq.cm. Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Anchorage dependency: Yes Memo: From lot.951101 by Minegishi,D. (transfer) [Pictures] Cell culture, Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Freezing process, (Date downloaded: 1999-08-09) |
