JCRB0609.1:Mm2T.P3

JCRB0609.1 [Mm2T.P3]

Profile:Mutntjac thymus cell cell line.

Date accepted: 06/09/2004

Previous cell Number: NIHS0358

Animal: indian muntjak

Sex and Age: M :1-day-old

Scientific Name: Numtiacus muntjak

Case history: normal

Classification: normal

Tissue prepared: thymus

Date cell culture started: --/--/1977

History of the cell: Cells were established by Takaoka,T. Culture condition was converted to serum free condition on 1991. Continuous cell culture was kept for many yeas.

Medium: Serum free DM201 medium.

Passage method: Cells are harvested by rubber policeman

Genetics: Choromosome analysis showes the cell line is derived from Muntjac. Chromosome number is 7. Telomere length is 7.7 kb.

Life span: infinite

Morphology: epithelial-like

Characteristics: Cells grew when started at 1977 but did not grow for 2 years after then. Cells were kept by changing medium for two years and finally cells started to grow again. Now cells are growing well. Medium was converted to serum free at 1991.

Established by Takaoka,T. and Katsuta,H.

Deposited by Takaoka,T.

Special notice: free

Cell bank: NIHS(JCRB)

References: (1)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Chromosome, Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

Additional Information(1)
Additional Information(2)
Additional Information(3)
Additional Information(4)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 07092004: seed (JCRB0609.1) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

87.6
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

LDH,NP,G6PD examined. Indian muntjak proved..

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

DM-201 medium. Serum free medium.

Freezing medium:

Special medium for freezing used. DM201 with FM-1 freezing medium (1 to 1 mix) with 10% DMSO.

Passage method:

Cells were harvested by scraper gently. Subculture every 4-6 days and medium change every 3-4 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

2-3 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasma test done by staining method(Hojo,M.) and PCR methods(Takada,Y.).

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

Freezing process,

Additional Information

(Date downloaded: 2005-09-30)

[ Lot No.: 07152004: seed (JCRB0609.1) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

89.3
Antibiotics used :Only MC210 temporary to remove mycoplasmas.

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD, NP, LDH examined. Indian muntjak confirmed..

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

DM201 medium. Serum free. (MC210 added only for 3 passages).

Freezing medium:

1 to 1 mixture of the culture medium and FM-1 medium containing 10% DMSO.

Passage method:

Cells were harvested with scraper. Subculture every 4-6 days and medium change every 3-4 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

2.0 x 10^4 cells/sq.cm.

[ Additional Data ]

Mycoplasma test,

Isozyme analysis,

Freezing process,

(Date downloaded: 2005-09-30)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Takaoka,T., Kasai,M., and Katsuta,H. A cell line derived from the Indian Muntjac thymus, Dokkyo J. Med. Sci., 9: 133-138, 1982.