| Date accepted: | 03/23/1985 |
| Animal: | human |
| Scientific Name: | Homo sapiens |
| Tissue prepared: | stomach |
| History of the cell: | 04/10/74 started |
| Medium: | RPMI1640 medium 45% with 45% Eagle's minimal essential medium and 10% heat inactivated FBS. |
| Passage method: | Simple dilution. |
| CO2 Concentration: | 5 % |
| Cell no. at passage: | 10^4 cells/ml |
| Genetics: | stomach cancer, signet ring cell carcinoma |
| Characteristics: | stomach cancer, signet ring cell carcinoma |
| Cell ID data: | available |
| DNA Profile (STR): | D5S818:10,11 D13S317:8,12 D7S820:8,12 D16S539:10,12 vWA:14,16 TH01:7,9 Amelogenin:X TPOX:11 CSF1PO:7,11 |
| Virus DNA detected: | CMV(-), EBV(-), HHV6(-), HHV7(-), BKJ(-), JCV(-), ADV1(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested] -- Description |
| Established by | Sekiguchi,M. |
| Deposited by | Sekiguchi,M. |
| Cell bank: | TIMS(JCRB) |
| References: | (1,2,3,4,5,6) |
(* The format of the table was revised on Apr.25.2003.)
| Cells(1), Mycoplasma test, Isozyme analysis, Chromosome, Chromosome, Chromosome, STR-PCR(1), STR-PCR(2), Freezing process, |
|
Additional Information(1) Additional Information(2) |
|
[ Lot No.: 061985: token/seed (JCRB0611) ] |
Date cell culture started:06/19/85 Concentration of cells in an ampule: 1 x 10^6 cell/ml Antibiotics used :KM No contamination with bacteria and fungi but cells were found to be contaminated with mycoplasmas. Decontamination under process. Tested Isoenzymes, AST,G6PD,(LD)*,MD,MPI,NP,PEPB/*only LD-B observed. Anchorage dependency: No [ Culture condition of this lot.]
Freezing medium: Culture medium with 5% DMSO Passage method: Simple dilution. Growth temperature: 37 C Cell No. at passage: 10^4 cells/ml [ Additional Data ]
(Date downloaded: 2007-11-15) |
|
[ Lot No.: : (JCRB0611) ] |
|
[ Culture condition of this lot.]
Passage method: dilution Growth temperature: 37 Cell No. at passage: 10^4/ml [ Additional Data ]
(Date downloaded: 2007-11-15) |
|
[ Lot No.: 950310: seed culture (JCRB0611) ] |
|
[ Culture condition of this lot.] [ Additional Data ]
(Date downloaded: 2007-11-15) |
|
[ Lot No.: 082995: seed (JCRB0611) ] |
Passage Number, P709 Cell culture was started without cloning from the previous stock. Date cell culture started:06/15/95 Concentration of cells in an ampule: 3.03x10^6 cell/ml Viability under microscope (%): 94.6 Antibiotics used :MC-210 treatment(0.5ug/ml)at 12.2 x 10^4 cells/ml. Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, LDH, NP, G6PD (human confirmed). Anchorage dependency: No Cell Identification: available [ Culture condition of this lot.]
Freezing medium: Culture medium with 5% DMSO Passage method: simple dilution Growth temperature: 37 C Cell No. at passage: 2.0x10^5 cells/ml Memo: from lot.061895 by Ohnishi/Okado [ Additional Data ]
(Date downloaded: 2007-11-15) |
|
[ Lot No.: 012296: distribution (JCRB0611) ] |
Passage Number, P712 Cell culture was started without cloning from the previous stock. Date cell culture started:01/09/96 Concentration of cells in an ampule: 2.4x10^6 cell/ml Viability under microscope (%): 94.0 Antibiotics used :Free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, NP, LDH, G6PD (human confirmed). Anchorage dependency: No [ Culture condition of this lot.]
Freezing medium: Culture medium with 5% DMSO. Passage method: Simple dilution. Growth temperature: 37 C Cell No. at passage: 2.0x10^5 cells/ml Memo: from lot.082995 by Nishimura,K. P709-P712/13 days. [ Additional Data ]
(Date downloaded: 2007-11-15) |
;){kind=link}
;){kind=link}
;){kind=link}
;){kind=link}
;){kind=link}
;){kind=link}
;){kind=link}
;){kind=link}
;){kind=link}
;){kind=link}
;){kind=link}
;){kind=link}