JCRB0648:L.P3

JCRB0648 [L.P3]

Profile:Serum free cell line of the mouse L929 cell line.

Date accepted: 10/08/1997

Animal: mouse

Strain: C3H

Scientific Name: Mus musclus

Case history: Cells were established by Katsuta,H. et.al. by serum free medium.

Classification: transformed

Tissue prepared: connective tissue

History of the cell: Takaoka,T.->Riken Cell Bank->JCRB

Medium: Although the DM160 medium was indicated but it can be replaced to the DM201 medium. Serum free. Frozen medium FM-1 established by Ohno,T. and destributed from Kyokuto Seiyaku Kogyo.Co. used.

Passage method: Harvest cells by rubber policeman.

CO₂ Concentration: 5 %

Cell no. at passage: 1 x 10^4 cells/sq.cm.

Genetics: skin

Life span: infinite

Morphology: epitherial-like

Characteristics: This cell line was surpplied from Riken Genebank for the replacement to the JCRB0605.

Established by Katsuta,H.

Deposited by Takaoka,T.

Special notice: free

Cell bank: NIHS(JCRB)

References: (1,2,3,4,5,6)

Comments: Using serum free medium all the time is essential.

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Freezing process, Others(1), Others(2), Others(6), Others(7),

[ Extra document(s) ]

Additional Information(1)
Additional Information(2)
Additional Information(3)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 112697: distribution (JCRB0648) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

82.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD, NP, LDH examined. Mouse confirmed..

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

DM160 medium only. No serum added.

Freezing medium:

Frozen medium FM-1 established by Ohno,T. and destributed from Kyokuto Seiyaku Kogyo.Co. used.

Passage method:

Cells are harvested with scraper (rubber policeman).

Growth temperature:

Cell No. at passage:

1 x 10^4 cells/sq.cm.

Memo:

[ Additional Data ]

Mycoplasma test,

Isozyme analysis,

Freezing process,

Additional Information

(Date downloaded: 2004-07-07)

[ Lot No.: 112597: seed (JCRB0648) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

84.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

DM160 medium only. No serum added.

Freezing medium:

Frozen medium FM-1 established by Ohno,T. and destributed from Kyokuto Seiyaku Kogyo.Co. used.

Passage method:

Cells are harvested with scraper (rubber policeman).

Growth temperature:

Cell No. at passage:

1 x 10^4 cells/sq.cm.

Memo:

[ Additional Data ]

Isozyme analysis,

Freezing process,

Additional Information

(Date downloaded: 2004-07-07)

[ Lot No.: 111197: seed (JCRB0648) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

85.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

DM160 medium only. No serum added.

Freezing medium:

Frozen medium FM-1 established by Ohno,T. and destributed from Kyokuto Seiyaku Kogyo.Co. used.

Passage method:

Cells are harvested with scraper (rubber policeman).

Growth temperature:

Cell No. at passage:

1 x 10^4 cells/sq.cm.

Memo:

[ Additional Data ]

Mycoplasma test,

Freezing process,

Additional Information

(Date downloaded: 2004-07-07)

Exported from cellroot.dbf and cellspec.dbf.

Reference List

1. Takaoka,T. Cell strains grown in protein and lipid free synthetic media, Soshiki Baiyo, 9: 196-200, 1983.

2. Katsuta,H. and Takaoka,T. Cultivation of cells in protein- and lipid-free synthetic media, Method. Cell Biol., 6: 1-42, 1973.

3. Kagawa,Y., Takaoka,T., and Katsuta,H. Absence of essential fatty acids in mammalian cell strains cultured in lipid-and protein-free chemically defined synthetic media, J. Biochem., 68: 133-136, 1970.

4. Ohno,T., Kurita,K., Abe,S., Eimori,N., and Ikawa,Y. A simple freezing medium for serum-free cultured cells., Cytotechnology, 1: 257-260, 1988.

5. Ohno,T. Not known, Saibou Kogaku, 7: 171-172, 1988.

6. Morita,H., Ysumitsu,H., Harada,H., Umeda,M., and Takaoka,T. Secretion of a novel cell-adhesive protein distinct from fibronectin by mouse L.P3 cells growing in protein- and lipit-free synthetic medium, Cell Struct. Funct., 13: 481-493, 1988.