| Date accepted: | 10/02/85 |
| Animal: | mouse |
| Scientific Name: | Mus musclus |
| Tissue: | peripheral blood |
| Medium: | RPMI1640 medium with 10% fetal calf serum |
| Passage method: | Suspend cells by pipetting. |
| Cell no. at passage: | 1-2 x10^5 cells/ml |
| Genetics: | myeloma |
| Characteristics: | myeloma |
| Established by | Yelton,D.E. et al. |
| Deposited by | Umeda,M. |
| Cell bank maintaining these cells. | YCBR(JCRB) |
| References: | * |
| Cell culture, Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Growth Curve, Freezing process, |
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No extradocument. |
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[ Lot No.:110885: token/seed (JCRB0708) ] |
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Date cell culture started:11/08/85 Concentration of cells in an ampule: 2x10^6/ml cell/ml Culture medium: RPMI1640 medium with 10% fetal calf serum. Growth temperature: 37 C Passage method: Suspend cells by pipetting. Cell No. at passage: 1-2 x10^5 cells/ml Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, AST,G6PD,LD,MD,MPI,NP,PEPB showed original animals derived. [Pictures]
(Date downloaded: 1999-08-11) |
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[ Lot No.:071095: token/seed (JCRB0708) ] |
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[Pictures]
(Date downloaded: 1999-08-11) |
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[ Lot No.:090997: seed (JCRB0708) ] |
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Date cell culture started:09/01/97 Concentration of cells in an ampule: 3.8x10^6 cell/ml Culture medium: RPMI1640 medium with 10% heat inactivated fetal calf serum (Mitsubishi PVF001). Growth temperature: 37 C Freezing medium: Culture medium with 5% DMSO. Passage method: Simple dilution. Cell No. at passage: 4x10^4 cells/ml Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, Under examination. showed original animals derived. Chromosome frequency distribution: Under examination. Anchorage dependency: No Memo: from 071095 by Kurematsu,M. (transfer). [Pictures] Cell culture, Mycoplasma test, Mycoplasma (PCR), Freezing process, (Date downloaded: 1999-08-11) |
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[ Lot No.:091097: distribution (JCRB0708) ] |
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Date cell culture started:09/01/97 Concentration of cells in an ampule: 3.8x10^6 cell/ml Culture medium: RPMI1640 medium with 10% heat inactivated fetal calf serum (Mitsubishi PVF001). Growth temperature: 37 C Freezing medium: Culture medium with 5% DMSO. Passage method: Simple dilution Cell No. at passage: 4x10^4 cells/ml Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, Under examination. showed original animals derived. Chromosome frequency distribution: Under examination. Anchorage dependency: No Memo: from 071095 by Kurematsu,M. (transfer). [Pictures] Cell culture, Mycoplasma test, Isozyme analysis, Growth Curve, Freezing process, (Date downloaded: 1999-08-11) |
