| Profile:Serum free rat ascited hepatoma cell line. Cells have been maintained by passage for long time. Extremely high passage number. |
| Date accepted: | 07/16/2004 |
| Other name: | JTC-16.P3 |
| Previous cell Number: | NIHS0368 |
| Animal: | rat |
| Sex: | M |
| Strain: | Donryu |
| Scientific Name: | Rattus norvegicus |
| Classification: | variant |
| Histological type of the cell: | hepatoma |
| Tissue prepared: | ascites |
| Medium: | DM210 medium. Serum free. |
| Passage method: | Cells are harvested with scraper |
| CO2 Concentration: | 5 % |
| Cell no. at passage: | 10^6 cells/ml |
| Life span: | infinite |
| Characteristics: | Ascites hepatoma, serum free cell culture. |
| Established by | Katsuta,H. & Takaoka,T. |
| Deposited by | Takaoka,T. |
| Cell bank: | NIHS(JCRB) |
| References: | (1,2,3) |
| Comments: | This cells were definitly adapted to the serum free condition for long time. Number of passages are extremely high. |
(* The format of the table was revised on Apr.25.2003.)
| Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Freezing process, Others(1), Others(2), |
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No extradocuments available. |
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[ Lot No.: 08042004: seed (JCRB0714.1) ] |
Passage Number, P4* Cell culture was started without cloning from the previous stock. Date cell culture started:07/16/2004 Concentration of cells in an ampule: 2.3 x 10^6 cell/ml Viability under microscope (%): 94.1 Antibiotics used :MC210 to remove mycoplasmas Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, G6PD, NP, LDH examined. rat... Anchorage dependency: Yes [ Culture condition of this lot.]
Freezing medium: 1 to 1 mix FM-1 medium and culture medium containing 10% DMSO(final). Passage method: Cells were harvested with scraper. Subculture every 5-7 days and medium change every 2-3 days. Growth temperature: 37 C CO2 concentration: 5 % Cell No. at passage: 2.0 x 10^6 cells/sq.cm. Additional comments: Mycoplasmas tested by PCR(Ozawa,Y.) and staining methods. [ Additional Data ]
(Date downloaded: 2005-09-30) |
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[ Lot No.: 08052004: distribution (JCRB0714.1) ] |
Passage Number, P4* Cell culture was started without cloning from the previous stock. Date cell culture started:07/16/2004 Concentration of cells in an ampule: 1.5 x 10^6 cell/ml Viability under microscope (%): 91.6 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, NP, LDH and G6PD examined. Rat,. Anchorage dependency: Yes [ Culture condition of this lot.]
Freezing medium: 1 to 1 mix FM-1 medium and culture medium containing 10% DMSO(final). Passage method: Cells were harvested with scraper. Subculture every 5-7 days and medium change every 2-3 days. Growth temperature: 37 C CO2 concentration: 5 % Cell No. at passage: 2.0 x 10^4 cells/sq.cm. Additional comments: Mycoplasmas tested by staining and PCR(Ozawa,Y.) methods. [ Additional Data ]
(Date downloaded: 2005-09-30) |
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