JCRB0718.1:RLC-10.P3

JCRB0718.1 [RLC-10.P3]

Profile:Rat normal cell line cultured with serum free medium for long passages.

Date accepted: 07/16/2004

Previous cell Number: NIHS0367

Animal: rat

Sex and Age: F :11-day-old

Strain: JAR-1

Scientific Name: Rattus norvegicus

Case history: normal

Classification: normal

Histological type of the cell: normal

Tissue prepared: liver

History of the cell: Takaoka,T. -> JCRB Cell Bank

Medium: Serum free DM201 medium.

Passage method: Cells were harvested by scraper gently and suspended in fresh medium.

Cell no. at passage: 1x10^6 cells/ml

Genetics: Telomelase was negative and a length of telomere was 2.0 Kbases.

Life span: infinite

Morphology: epithelial-like

Established by Takaoka,T.

Deposited by Takaoka,T.

Special notice: free

Cell bank: NIHS(JCRB)

References: (1,2,3,4)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Freezing process,

[ Extra document(s) ]

Additional Information(1)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 08262004: seed (JCRB0718.1) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

75.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

Freezing medium:

1 to 1 mixture of the culture medium and the FM-1 medium containing final 10% DMSO.

Passage method:

Cells were harvested with scraper and changed medium every 7-10 days. Medium change was done every 2-4 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

1.1 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasma test done by staining(Kurematsu,M.) and PCR(Ozawa,Y.) method.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Freezing process,

(Date downloaded: 2004-11-09)

[ Lot No.: 09222004: distribution (JCRB0718.1) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

78.6
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

DM201 medium. Serum free.

Freezing medium:

1 to 1 mixture of the culture medium and the FM-1 medium containing final 10% DMSO.

Passage method:

Cells were harvested with scraper, subculture 7-10 days. Medium change every 2-3 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

1.1 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasmas tested by staining(Kurematsu,M.) and PCR(Ozawa,Y.) methods.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Freezing process,

(Date downloaded: 2004-11-09)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Katsuta,H. and Takaoka,T. Protein- and lipid-free synthetic media for the cultivation of mammalian cells in tissue culture. In: H.Katsuta (ed.), <[13] ,Page Numbers>Tokyo: Jpn.Sci.Soc.Press. 1978
. Notes: Our investigation on the nutritional requirements of cells in tissue culture commenced with the cultivation of rat ascites hepatoma AH-130 cells. The content of nitrogen in media and cells was determined before and after the cultivation, but little decrease was found in the contents during several days of culture. The incorporation of 131I-labeled serum proteins into cells during cultuvation was estimated. No significant incorporation, however, was confirmed........
Reference ID: 158
2. Katsuta,H., Takaoka,T., and Yasumoto,S. Toxic metabolites released from rat hepatoma cells in culture. I. effects of metabolites of hepatomas on various cells, J. Natl. Cancer Inst., 51: 1841-1844, 1973.
Notes: All fractions obtained with Sephadex G-10 or G-25 form media in which various rat hepatoma cells were cultued inhibited the growth of normal rat liver cells accelerated growth. The fraction from the media of rat hepatomas had little effect on the growth of rat hepatoma cells but markedly inhibited growth of liver cells which were not backtransplantable.
Reference ID: 1710
3. Katsuta,H. and Takaoka,T. Carcinogenesis in tissue culture. XIV. Malignant transformation of rat liver parenchymal cells treated with 4-nitroquinoline 1- oxide in tissue culture, J. Natl. Cancer Inst., 49: 1563-1576, 1972.
Reference ID: 2902
4. Yamada,T., Takaoka,T., and Katsuta,H. Carcinogenesis in Tissue Culture, 23:Population analysis in the cultures of transformed rat liver cells by cell electrophoresis, Carcinogenesis in Tissue Culture, 1974.
Notes: Origin of this papaer is not clear. Data entered by a incomplete copy of the document.
Abstract: In eight cultured strains of rat liver cells transformed in culture with 4-nitrozuinoline 1-oxide (4NQO) or spontaneously, population analysis of cells was carried out by an automatic photo-recording cell electrophoresis. The lowest frequency range of the presence of malignant transformants in the cell strains was anticipated to be less than 5%, and the cell strains having low population of malignant transformants were those transformed spontaneously or by a single or several times of application of 4NQO. On the contrary, the highest was as many as 18%, the strains malignantly transformed in culture by manly times of application represented higher population of trnasformants. Hence it was strongly suggested that, when cell strains were malignantly transformed in culture by different ways, their malignant cell populations were quite different in number from each other. The biological property of cell strains transformed in culture could be not reasonably compared with each other unless cell population analysis is employed.
Reference ID: 5572