JCRB0834:NUGC-4

JCRB0834 [NUGC-4]

Date accepted:	03/30/1993
Animal:	human
Sex and Age:	F :35-year-old
Scientific Name:	Homo sapiens
Case history:	poorly differ. adenocarcinoma
Classification:	tumor
Tissue prepared:	gastric lymph node
Origin of tumor:	gastric lymph node
Metastatic tissue:	lymph node
Date cell culture started:	05/01/82
History of the cell:	Deposited by the original investigator.
Medium:	RPMI1640 medium with 10% fetal calf serum.
Passage method:	Simple dilution.
CO₂ Concentration:	5 %
Cell no. at passage:	2.0x10^5 cells/ml
Life span:	infinite
Morphology:	spherical
Characteristics:	signet ring cell carcinoma
Cell ID data:	available
DNA Profile (STR):	D5S818:11,12 D13S317:11,13 D7S820:11 D16S539:9 vWA:14,18 TH01:7,9 Amelogenin:X TPOX:8,9 CSF1PO:10,11
Virus DNA detected:	CMV(-), EBV(-), HHV6(-), HHV7(-), BKJ(-), JCV(-), ADV1(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested] -- Description
Established by	Akiyama,S.
Deposited by	Akiyama,M.
Cell bank:	RERF(JCRB)
References:	(1,2)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Mycoplasma test, Mycoplasma (PCR), STR-PCR(1), STR-PCR(2), Growth Curve, Freezing process, Others(1),

[ Extra document(s) ]

Additional Information(1)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 000000: distribution (JCRB0834) ]

[ Culture condition of this lot.]

Culture medium:

No culture information received at a moment. Please contact to Dr.Akiyama of RERF.

[ Additional Data ]

(Date downloaded: 2007-11-15)

[ Lot No.: 950904: token/seed (JCRB0834) ]

Date cell culture started:

[ Culture condition of this lot.]

[ Additional Data ]

(Date downloaded: 2007-11-15)

[ Lot No.: 031097: seed (JCRB0834) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Growth rate:

Viability under microscope (%):

93.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

RPMI1640 medium with 10% heat inactivated fetal calf serum (Mitsubishi PVF01).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

simple dilution

Growth temperature:

Cell No. at passage:

2.1x10^5 cells/ml

Memo:

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

STR-PCR(1),

STR-PCR(2),

Growth Curve,

Freezing process,

(Date downloaded: 2007-11-15)

[ Lot No.: 031197: distribution (JCRB0834) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Growth rate:

Viability under microscope (%):

93.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

RPMI1640 medium with 10% heat inactivated fetal calf serum (Mitsubishi PVF01).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

simple dilution

Growth temperature:

Cell No. at passage:

2.1x10^5 cells/ml

Memo:

[ Additional Data ]

Mycoplasma test,

Freezing process,

(Date downloaded: 2007-11-15)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Koyama,K., Akiyama,K., Kawahara,H., Egashira,A., and Murakami,H. Alloimunization with cultured human stomach cancer cell lines and the establishment of human-human hybridomas producing monoclonal antibodies, Jpn. J. Cancer Res., 81: 967-970, 1990.

2. Akiyama,S., Amo,H., Watanabe,T., Matsuyama,M., Sakamoto,J., Imaizumi,M., Ichihashi,H., Kondo,T., and Takagi,H. Characteristics of three human gastric cancer cell lines, NU-GC-2 NU-GC-3 and NU-GC-4, Jpn. J. Surg., 18: 438-446, 1988.