Passage Number, NA
PDL, NA
Cell culture was started without cloning from the previous stock.
Date cell culture started:08/19/2003
Concentration of cells in an ampule: NA cell/ml
Viability under microscope (%): NA
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, G6PD(human type B), NP, LD examined. Not HeLa..
Anchorage dependency: Yes

Culture medium: DMEM with 10% FBS
Freezing medium: not done. discarded
Passage method: 0.1% trypsin and 0.02% EDTA
Growth temperature: 37 C
CO₂ concentration: 5 %
Cell No. at passage: 8.0 x 10^3 cells/sq.cm.
Memo: Condition of cells were not good and discarded. Cells were overgrown because of the timing of the subculture was lost.
Additional comments: Mycoplasmas tested by PCR(Takada,Y.) method.

[ Additional Data ]

Cells(1), Cells(2), Cells(3), Mycoplasma (PCR), Isozyme analysis, Chromosome, Chromosome, Chromosome, Chromosome,

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(Date downloaded: 2004-07-15)

Tests for mycoplasma, bacteria, and fungi were negative.
Anchorage dependency: Yes

CO₂ concentration: 5 %
Additional comments: Mycoplasmas tested by PCR (Takada,Y.) methods.

[ Additional Data ]

Mycoplasma (PCR),

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(Date downloaded: 2004-07-15)

Cells are propagated from the stock, 10082002
Passage Number, P209
Cell culture was started without cloning from the previous stock.
Date cell culture started:03/17/2004
Concentration of cells in an ampule: 2.6 x 10^6 cell/ml
Viability under microscope (%): 96.6
Antibiotics used :free
Sterility test has not completed yet.
Anchorage dependency: Yes

Culture medium: Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Mitsubishi Co. PVF01.).
Freezing medium: Culture medium containing 5% DMSO.
Passage method: Cells harvested after treatment with 0.1% trypsin and 0.02% EDTA at room temperature for three minutes.
Growth temperature: 37 C
CO₂ concentration: 5 %
Cell No. at passage: 1.0 x 10^4 cells/sq.cm.

[ Additional Data ]

Freezing process,

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(Date downloaded: 2004-07-15)

Cells are propagated from the stock, 10182002
Passage Number, P209
Cell culture was started without cloning from the previous stock.
Date cell culture started:03/17/2004
Concentration of cells in an ampule: 2.6 x 10^6 cell/ml
Viability under microscope (%): 96.6
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, G6PD(typeB), NP, LDH examined. Human, not HeLa..
Anchorage dependency: Yes
Cell Identification: available
RFLP data: Cells ID was unique and consistent with other OUMS series cell lines.

Culture medium: Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Mitsubishi Co. PVF01).
Freezing medium: Culture medium with 5% DMSO.
Passage method: Cells harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Growth temperature: 37 C
CO₂ concentration: 5 %
Cell No. at passage: 1.0 x 10^4 cells/sq.cm.
Additional comments: Mycoplasmas tested by PCR(Takada,Y) and staining methods.

[ Additional Data ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, STR-PCR(1), STR-PCR(2), Freezing process,

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(Date downloaded: 2004-07-15)