JCRB1006.3:OUMS-36T-3

JCRB1006.3 [OUMS-36T-3]

Profile:hTRT gene transfected normal human embryo fibroblast cell line.

Date accepted: 01/30/2001

Previous cell Number: NIHS0291.3

Animal: human

Race: Japanese

Scientific Name: Homo sapiens

Case history: normal transfected with hTRT gene

Classification: transformed

Tissue prepared: embryo

History of the cell: Original developer -> JCRB

Medium: Dulbecco's modified Eagle's medium with 10% fetal bovine serum.

Passage method: 0.1% trypsin and 0.02% EDTA.

CO₂ Concentration: 5 %

Genetics: 10^6 cells/well were transfected with pMXpuro-hTRT (100ng/ml) twice with electroporation.

Life span: infinite

Morphology: fibroblast-like

Characteristics: normal human embryo cells transfected by hTRT gene (pMXpuro).

Cell ID data: available

DNA Profile: D5S818:10,12
D13S317:8,11
D7S820:11
D16S539:9,10
vWA:18,20
TH01:6,9
Amelogenin:X
TPOX:8
CSF1PO:12

Established by Kouchi,Y. & Namba,M.

Deposited by Namba,M.

Cell bank: NIHS(JCRB)

References: (1)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Chromosome, Chromosome, Chromosome, Chromosome, Chromosome, Chromosome, STR-PCR(1), STR-PCR(2), Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

No extradocuments available.

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 06262003: seed (JCRB1006.3) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

90.4
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(typeB), NP, LDH examined. Human, not HeLa..

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium with 10% fetal bovine serum.

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells harvested after treatment with 0.1% trypsin and 0.02% EDTA at room temperature for three minutes.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

7.7 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasmas tested by PCR(Takada,Y.) method.

[ Additional Data ]

Mycoplasma (PCR),

Isozyme analysis,

Chromosome,

Chromosome,

Chromosome,

Chromosome,

Chromosome,

Chromosome,

STR-PCR(1),

STR-PCR(2),

Freezing process,

(Date downloaded: 2003-10-09)

[ Lot No.: 06272003: distribution (JCRB1006.3) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

90.4
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

Under examination..

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium with 10% fetal bvine serum (Mitsubishi, PVF01).

Freezing medium:

Culture medium containing 5% DMSO.

Passage method:

Cells are harvested after treatment with 0.1 % trypsin and 0.02% EDTA at room temperature for 3 minutes. Subculture once a week and medium change once a week.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

9.1 x 10^3 cells/sq.cm.

Additional comments:

Mycoplasmas tested by staining(Kurematsu,M.).

[ Additional Data ]

Mycoplasma test,

Freezing process,

(Date downloaded: 2003-10-09)

Exported from cellroot.dbf and cellspec.dbf.

Reference List

1.Kouchi,H. and Namba,M. Normal huma fibroblast cell lines tranfected with the hTRT gene -OUMS36T cell line series-, Tiss. Cult. Res. Commun., 19: 203-204, 2000.