Passage Number, P44
Cell culture was started without cloning from the previous stock.
Date cell culture started:02/26/2004
Concentration of cells in an ampule: 3.1 x 10^6 cell/ml
Viability under microscope (%): 98.1
Antibiotics used :free
Tests for mycoplasma, bacteria, and fungi were negative.
Tested Isoenzymes, G6PD(human type B), NP, LD examined. No HeLa..
Anchorage dependency: Yes
Cell Identification: available
RFLP data: This cell line is identified to be unique with the STR-PCR experiment.

Culture medium: Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Mitsubishi PVF01).
Freezing medium: Culture medium with 5% DMSO.
Passage method: Cells were harvested after treatment with 0.1% typsin and 0.02% EDTA at room temperature for three minutes.
Growth temperature: 37 C
CO₂ concentration: 5 %
Cell No. at passage: 7.5 x 10^3 cells/sq.cm.
Additional comments: Mycoplasmas tested by staining(Kurematsu,M.) and PCR(Takada,Y.) methods.

[ Additional Data ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Chromosome, Chromosome, Chromosome, Chromosome, STR-PCR(1), STR-PCR(2), Freezing process,

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(Date downloaded: 2004-04-01)

Cells are propagated from the stock, 12122002
Passage Number, P44
Cell culture was started without cloning from the previous stock.
Date cell culture started:02/26/2004
Concentration of cells in an ampule: 3.1 x 10^6 cell/ml
Viability under microscope (%): 98.1
Antibiotics used :free
Sterility test has not completed yet.
Anchorage dependency: Yes

Culture medium: Dulbecco's modified Eagle's medium with 10% fetal bovine serum(Mitsubishi PVF01).
Freezing medium: Culture medium with 5% DMSO.
Passage method: Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA at room temperature for three minutes.
Growth temperature: 37 C
CO₂ concentration: 5 %
Cell No. at passage: 7.5 x 10^3 cells/sq.cm.

[ Additional Data ]

Freezing process,

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(Date downloaded: 2004-04-01)