JCRB1006.5F:OUMS-36T-5F

JCRB1006.5F [OUMS-36T-5F]

Date accepted:	02/17/2002
Previous cell Number:	NIHS0291.5S
Animal:	human
Race:	Japanese
Scientific Name:	Homo sapiens
Case history:	normal
Classification:	transformed
Histological type of the cell:	normal cells transfected with hTRT gene
Tissue prepared:	embryo
History of the cell:	Original developer -> JCRB
Medium:	Dulbecco's modified Eagle's medium with 10% fetal bovine serum.
Passage method:	0.1% trypsin and 0.02% EDTA.
CO₂ Concentration:	5 %
Life span:	infinite
Morphology:	fibroblast-like
Characteristics:	P196
Cell ID data:	available
DNA Profile:	D5S818:10,12 D13S317:8,11 D7S820:11 D16S539:9,10 vWA:18,21 TH01:6,9 Amelogenin:X TPOX:8 CSF1PO:12
Established by	Kouchi,Y. & Namba,M.
Deposited by	Namba,M.
Cell bank:	NIHS(JCRB)
References:	(1)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, STR-PCR(1), STR-PCR(2), Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

No extradocuments available.

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 04102003: seed (JCRB1006.5F) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

97.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(typeB),NP,LDH examined. Human, not HeLa..

Anchorage dependency:

Cell Identification:

RFLP data:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium with 10% fetal calf serum.

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells were harvested after treatment with 0.1% trypsin.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

1.0 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasmas tested by staining(Kurematsu,M.) and PCR(Takada,Y.) methods. Negative at 3 months' continuous culture after the decontamination.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

STR-PCR(1),

STR-PCR(2),

Freezing process,

(Date downloaded: 2003-07-29)

Exported from cellroot.dbf and cellspec.dbf.

Reference List

1.Kouchi,H. and Namba,M. Normal huma fibroblast cell lines tranfected with the hTRT gene -OUMS36T cell line series-, Tiss. Cult. Res. Commun., 19: 203-204, 2000.