JCRB1006.6F:OUMS-36T-6F

JCRB1006.6F [OUMS-36T-6F]

Date accepted:	02/17/2002
Previous cell Number:	NIHS0291.6S
Animal:	human
Race:	Japanese
Scientific Name:	Homo sapiens
Case history:	normal transfected with hTRT gene
Classification:	transformed
Tissue prepared:	embryo
History of the cell:	Original developer -> JCRB
Medium:	Dulbecco's modified Eagle's medium with 10% fetal bovine serum.
Passage method:	0.1% trypsin and 0.02% EDTA.
CO₂ Concentration:	5 %
Life span:	infinite
Morphology:	fibroblast-like
Characteristics:	P210
Cell ID data:	available
DNA Profile (STR):	D5S818:10,12 D13S317:8,11 D7S820:11 D16S539:9,10 vWA:18,20 TH01:6,9 Amelogenin:X TPOX:8 CSF1PO:12
Virus DNA detected:	CMV(-), EBV(-), HHV6(-), HHV7(-), BKJ(-), JCV(-), ADV1(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested] -- Description
Established by	Kouchi,Y. & Namba,M.
Deposited by	Namba,M.
Cell bank:	NIHS(JCRB)
References:	(1)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, STR-PCR(1), STR-PCR(2), Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

Additional Information(1)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 06042003: seed (JCRB1006.6F) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

99.6
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(typeB),NP,LDH examined. Human, not HeLa..

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium with 10% Fetal calf serum (Mitsubishi PFCS PVF001).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells harvested after treatment with 0.1% typrsin. Subculture twice a week and change medium every 3-4 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

2.1 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasmas tested by staining(Kurematsu,M.) and PCR(Takada,Y.) methods.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

STR-PCR(1),

STR-PCR(2),

Freezing process,

(Date downloaded: 2007-11-15)

[ Lot No.: 06052003: distribution (JCRB1006.6F) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

99.6
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(typeB),NP,LDH examined. Human, not HeLa..

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium with 10% fetal calf serum(Mitsubishi PFCS PVF001).

Freezing medium:

Culture medium with 10% fetal calf serum.

Passage method:

Cells harvested after treatment with 0.1% trypsin. Subculture twice a week. Medium change every 3 - 4 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

2.1 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasmas tested by staining(Kurematsu,M.) method.

[ Additional Data ]

Mycoplasma test,

Isozyme analysis,

Freezing process,

(Date downloaded: 2007-11-15)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Kouchi,H. and Namba,M. Normal huma fibroblast cell lines tranfected with the hTRT gene -OUMS36T cell line series-, Tiss. Cult. Res. Commun., 19: 203-204, 2000.