JCRB1074:FU97

JCRB1074 [FU97]

Profile:Human stomach cancer cell line with AFP production.

Date accepted: 07/31/2003

Previous cell Number: NIHS0332

Animal: human

Sex and Age: F :66-year-old

Race: Japanese

Scientific Name: Homo sapiens

Case history: stomach cancer, 2266ng/ml AFP observed before the operation. Lymph node metastasis and pancreatic metastasis observed. ss, ssINFbehta, ly3, v0.

Classification: tumor

Histological type of the cell: poorly differentiated adenocarcinoma.

Tissue prepared: stomach

Origin of tumor: stomach

Date cell culture started: 09/12/1994

History of the cell: Osaka Prefecture University -> JCRB

Medium: Dulbecco's modified Eagle's medium(4.5g/L glucose) with 10% fetal calf serum and 10mg/L insulin.

Passage method: Cells are harvested after treatment with trypsin-EDTA.

CO₂ Concentration: 5 %

Life span: infinite

Morphology: epithelial-like

Characteristics: Td = 95 hrs. AFP production and tumorigenesity is maintained in the cell line.

Cell ID data: available

DNA Profile: D5S818:
D13S317:
D7S820:
D16S539:
vWA:
TH01:
Amelogenin:
TPOX:
CSF1PO:

Established by Matsuda,M.

Deposited by Iwai,M.

Cell bank: NIHS(JCRB)

References: (1,2)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), STR-PCR(1), STR-PCR(2), Freezing process, Others(1),

[ Extra document(s) ]

No extradocuments available.

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 02052004: distribution (JCRB1074) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

97.9
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium (High glucose) with 10% fetal calf serum (Intergen RB52305) and 10mg/L insulin.

Freezing medium:

Culture medium with 5 % DMSO.

Passage method:

Cells harvested after treatment with 0.3% trypsin. Subculture once 5-7 days and medium change every 2-3 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

8.8 x 10^3 cells/sq.cm.

Additional comments:

Mycoplasmas tested by staining(Kurematsu,M.) and PCR(Takada,Y.) methods.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Freezing process,

(Date downloaded: 2004-03-02)

[ Lot No.: 02022004: seed (JCRB1074) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

96.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium (high glucose) with 10% fetal calf serum(Intergen RB52305) and 10mg/L insuline.

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells harvested after treatment with 0.3% trypsin. Subculture once every 5-7 days and medium change every 2-3 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

4.0 x 10^3 cells/sq.cm.

Additional comments:

Mycoplasmas tested by staining(Kurematsu,M.) and PCR(Takada,Y.) methods.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

STR-PCR(1),

STR-PCR(2),

Freezing process,

(Date downloaded: 2004-03-02)

Exported from cellroot.dbf and cellspec.dbf.

Reference List

1.Matsuda,M., Watanabe,A., Sawada,H., Yamada,Y., Nakano,H., Iwai,M., and Iwai,Y. Establishment of an alpha-fetoprotein-producing cell line derived from gastric cancer, In Vitro Cell Dev. Biol. -Animal, 35: 555-557, 1999.

2.Matsuda,M. Biological behavior of an alpha-fetoprotein-producing gastric cancer cell line (FU97), J. Nara Med. Ass., 51: 79-89, 2000.