JCRB1075.1:IM95m

JCRB1075.1 [IM95m]

Profile:Human gastric cancer cell line.

Date accepted: 07/31/2003

Previous cell Number: NIHS0334

Animal: human

Sex and Age: M :63-year-old

Race: Japanese

Scientific Name: Homo sapiens

Case history: moderately differentiated adenocarcinoma of stomach

Classification: tumor

Histological type of the cell: stomach cancer

Tissue prepared: stomach

Origin of tumor: stomach

Date cell culture started: --/--/1995

History of the cell: Osaka Prefecture University -> JCRB

Medium: Dulbecco's modified Eagle's medium(4.5g/L glucose) with 10% fetal bovine serum and 10mg/L insulin.

Passage method: Cells were harvested after the treatment with trypsin-EDTA.

CO₂ Concentration: 5 %

Life span: infinite

Morphology: epithelial-like

Characteristics: Clone from the IM95. A clone from the IM95, produces higher level of HGF & VEGF.

Cell ID data: available

DNA Profile: D5S818:9,11
D13S317:10,12
D7S820:10
D16S539:9,10
vWA:17,18
TH01:6,10
Amelogenin:X
TPOX:8,10
CSF1PO:11

Established by Iwai,M. & Matsuda,M.

Deposited by Iwai,M.

Cell bank: NIHS(JCRB)

References: (1,2,3)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), STR-PCR(1), STR-PCR(2), STR-PCR(3), Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

Additional Information(1)

[ ]

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 10282003: seed (JCRB1075.1) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

98.8
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium with 10% fetal calf serum (Intergen RB52305).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells were harvested after treatment with 0.3% trypsin. Subculture every 5-7 days. Medium change every 2-3 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

1.0 x 10^5 cells/sq.cm.

Additional comments:

Adhesion looks very weak and make clusters when cells grow. Mycoplasmas tested by PCR method(TY).

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Freezing process,

(Date downloaded: 2004-06-01)

[ Lot No.: 02122004: seed (JCRB1075.1) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

99.0
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium with high glucose concentration and 10% fetal calf serum (Intergen RB52305).

Freezing medium:

Culture medium containing 5% DMSO.

Passage method:

Cells harvested after treatment with 0.3% trypsin, subculture every 5-7 days and medium change every 2-3 days.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

5.0 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasmas tested by saining(Kurematsu,M.) and PCR(Takada,Y.) methods.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

STR-PCR(1),

STR-PCR(2),

STR-PCR(3),

Freezing process,

(Date downloaded: 2004-06-01)

Exported from cellroot.dbf and cellspec.dbf.

Reference List

1.Iwai,Y., Iwai,M., Matsuda,M., Watanabe,A., Nakano,H., and Imai,S. Establishment of human gastric cancer cell ine, Abstract of the Cancer Meeting, Japan., 1301, 1995.

2.Iwai,M. and Matsuda,M. Cloning of HGF-producing cell derived from human gastric cancer cells., Abstruct of the Cancer Meeting, Japan, 3487 PA, 2003.

3.Iwai,M., Matsuda,M., and Iwai,Y. Cloning of a cancer cell-producing hepatocyte growth factor, vascular endothelial growth factor, and interleukin-8 from gastric cancer cells, In Vitro Cell Dev. Biol. Anim, 39: 288-290, 2003.