JCRB1079:IHH-4

JCRB1079 [IHH-4]

Profile:Human papillary thyroid carcinoma cell line metastased to lymphnode. STR-PCR analysis showed the same pattern with the cell line TMH-1 (see CellID section).

Date accepted: 02/24/2001

Previous cell Number: NIHS0270

Animal: human

Sex and Age: M :75-year-old

Race: Japanese

Scientific Name: Homo sapiens

Case history: A papillary thyroid carcinoma identified originally and pulmonary metastases identified later.

Classification: tumor

Tissue prepared: lymph node

Origin of tumor: thyroid

Metastatic tissue: lymph node

History of the cell: Cells were deposited directly form the original developper.

Medium: 1 to 1 mixture of Dulbecco's modified Eagle's medium and RPMI1640 medium with 10% heat inactivated fetal bovine serum.

Passage method: Cells are harvested after treatment with 0.25% trypsin.

CO₂ Concentration: 5 %

Cell no. at passage: 10^3 cells/ml

Genetics: Doubling time = 42 hrs, Chromosome number = 41 - 47 (46).

Life span: infinite

Morphology: small polygonal shaped

Characteristics: Original tumor appeared in thyroid. Cells are adherent monolayer with small polygonal shaped. Multidrug registance.

Cell ID data: available

DNA Profile: D5S818:9,13
D13S317:10,13
D7S820:9,10
D16S539:9,11
vWA:16,18
TH01:9
Amelogenin:XY
TPOX:11
CSF1PO:12

Established by Sekiguchi,M. and et. al.

Deposited by Sekiguchi,M.

Special notice: Not identified.

Cell bank: NIHS(JCRB)

References: (1)

Comments: Cross contamination with TMH-1(NIHS0267) is suspected.

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Chromosome, Chromosome, Chromosome, Chromosome, Chromosome, Chromosome, STR-PCR(1), STR-PCR(2), Freezing process, Others(1), Others(2), Others(5), Others(6), Others(7), Others(8)

[ Extra document(s) ]

No extradocuments available.

[ ]

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 12042003: seed (JCRB1079) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

99.5
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium + RPMI1640 medium (1 to 1 mix) with 10% heat inactivated fetal bovine serum (RB52305).

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells were harvested after treatment with 0.25% trypsin.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

7.0 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasmas tested by staining and PCR method.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Chromosome,

Chromosome,

Chromosome,

Chromosome,

Chromosome,

Chromosome,

STR-PCR(1),

STR-PCR(2),

Freezing process,

(Date downloaded: 2004-07-15)

[ Lot No.: 12052003: distribution (JCRB1079) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

99.5
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD(typeB), NP, LDH examined. Human but not HeLa..

Anchorage dependency:

Cell Identification:

RFLP data:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium + RPMI1640 medium (1 to 1 mix) with 10% heat inactivated fetal bovine serum (Intergen RB52305).

Freezing medium:

Culture medium containing 5% DMSO.

Passage method:

Cells harvested after treatment with 0.25% trypsin.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

7.1 x 10^4 cells/sq.cm.

Additional comments:

Mycoplasmas tested by staining and PCR method.

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

STR-PCR(1),

STR-PCR(2),

Freezing process,

(Date downloaded: 2004-07-15)

Exported from cellroot.dbf and cellspec.dbf.

Reference List

1. Sekiguchi,M., Shiroko,Y., Arai,T., Kishino,T., Sugawara,I., Kudakabe,T., Suzuki,T., Yamashita,T., Obara,T., Ito,K., and Hasumi,K. Biological characteristics and chemosensitivity profile of four human anaplastic thyroid carcinoma cell lines, Biomed. Pharmacother., 55: 466-474, 2001.