JCRB1213.3:DIG104.10H.1

JCRB1213.3 [DIG104.10H.1]

Profile:Mouse hybridoma cell line producing monoclonal antibody against digoxin.

Date accepted: 09/28/2007

Previous cell Number: NIHS0562.3

Animal: mouse x mouse

Scientific Name: hybridoma

Classification: hybridoma

Histological type of the cell: producing Mab digoxin.

Medium: Dulbecco's modified Eagle's medium with 10% fetal bovine serum

Passage method: dilution

CO₂ Concentration: 5%

Cell no. at passage: split 1/5 (1/2 - 1/10)

Life span: infinite

Morphology: lymphocyte-like

Characteristics: Producing anti Digoxin IgG1 (k) isotype (RIA 120/130000)

Established by Sawada,J.

Deposited by Sawada,J.

Cell bank: NIHS(JCRB)

References: (1,2,3)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Chromosome, Chromosome,

[ Extra document(s) ]

No extradocuments available.

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 11052007: distribution (JCRB1213.3) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

76.7
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

DMEM with 10% heat inactivated fetal bovine serum(Hyclone GRD0051)

Freezing medium:

Cell Banker BLC-1(Nihon Zenyaku Industries)

Passage method:

dilution 1/5 (1/2-1/10)

Growth temperature:

CO₂ concentration:

Cell No. at passage:

1.0x10^5 cells/ml

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

(Date downloaded: 2008-01-21)

[ Lot No.: 11022007: seed (JCRB1213.3) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

56.7
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Kariology:

Chromosome frequency distribution:

<91/13 92/3 93/11 94/4 95/12 96/4 98/2 100/1 (50)

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

DMEM with 10% heat inactivated fetal bovine serum(Hyclone GRD0051)

Freezing medium:

Cell Banker BLC-1(Nihon Zenyaku Industries)

Passage method:

dilution 1/5 (1/2-1/10)

Growth temperature:

CO₂ concentration:

Cell No. at passage:

1.0x10^5 cells/ml

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Chromosome,

Chromosome,

(Date downloaded: 2008-01-21)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Yoshimatsu,K., Satake,M., Shimomura,K., Sawada,J.I., and Terao,T. Determination of cardenolides in hairy root cultures of Digitalis lanata by enzyme-linked immunosorbent assay, J. Nat. Prod., 53: 1498-1502, 1990.

2. Yoshimatsu,K., Sawada,J., Jaziri,M., and Shimomura,K. Detection of cardenolides by ELISA in plant sciences. In: Anonymous<[13] ,Page Numbers>Elsevier Science B.V. 1995
. Notes: Cardernolides are composed of a steroid or an aglycone porion which contains a five membered lactone ring and from one to four sugar molecules. They naturally occur among several plant families and some of them such as digoxin, lanatoside C and deslanoside derived from genus Digitalis (Scrophulariaceae) are frequently prescribed for congestive heart failure. ...
Reference ID: 6205
3. Sawada,J., Janejai,N., and Terao,T. Prodution and characterization of monoclonal and polyclonal antibodyies against digoxin, Bull. Natl. Inst. Hyg. Sci., 108: 29-33, 1990.
Notes: Three hybridoma cell lines (DIG 64.2B.5, DIG 104.H10 and DIG222.4D.5) producing monoclonal antibodyies against digoxin were established, and the properties of these monoclonal antibodyies were characterized and comared with three polyclonal rabblit anti-digoxin antisera....
Reference ID: 6206