JCRB1221:NCC-RbC-92

JCRB1221 [NCC-RbC-92]

Profile:Huaman retinoblastoma cell line.

Date accepted: 05/18/2006

Previous cell Number: NIHS0504

Animal: human

Sex and Age: F :1.0-year-old

Race: Japanese

Scientific Name: Homo sapiens

Case history: Primary unilateral retinoblastoma

Classification: tumor

Tissue prepared: primary retinoblastoma

Medium: CMRL1066 medium with 10-15% fetal bovine serum (100 μM Glutamine, 200 μM Sodium pyruvate and 20 μM 2-mercaptoetanol)

Passage method: Simple dilution. split ratio=1/5. Subculture every 1 week.

CO₂ Concentration: 5%

Cell no. at passage: 8 x 10^5 cells / mL

Genetics: Unilateral retinoblastoma

Life span: infinite

Morphology: lymphoblast-like

Cell ID data: available

DNA Profile (STR): D5S818:10,11
D13S317:8,10
D7S820:11,12
D16S539:9,11
VWA:18,20
TH01:6,7
Amelogenin:X
TPOX:8,11
CSF1PO:10,13

Virus DNA detected: GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested, GAPDH is a control] -- Description

Established by Inomata, M.

Deposited by Inomata, M.

Cell bank: NIHS(JCRB)

References: (1)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Chromosome, Chromosome, STR-PCR(1), STR-PCR(2),

[ Extra document(s) ]

No extradocuments available.

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 02192008: distribution (JCRB1221) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

CMRL1066 medium(GIBCO) with 100uM Glutamine ,200uM Sodium pyruvate,50uM 2-Mercaptoetanol and 15% heat inactivated fetal bovine serum(Hyclone GRD0051)

Freezing medium:

Cell Banker BLC-1(Nihon Zenyaku Industries)

Passage method:

dilution,split 1/2

Growth temperature:

CO₂ concentration:

Cell No. at passage:

2.4x10^5 cells/ml

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

STR-PCR(1),

STR-PCR(2),

(Date downloaded: 2008-08-27)

[ Lot No.: 02052008: seed (JCRB1221) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

Tests for mycoplasma, bacteria, and fungi were negative.

Kariology:

Chromosome frequency distribution:

<64/9 67-83/9 77-92/8 95/19 96/1 >97/4 (50)

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

CMRL1066 medium(GIBCO) with 100uM Glutamine ,200uM Sodium pyruvate,50uM 2-Mercaptoetanol and 15% heat inactivated fetal bovine serum(Hyclone GRD0051)

Freezing medium:

Cell Banker BLC-1(Nihon Zenyaku Industries)

Passage method:

dilution,split 1/2

Growth temperature:

CO₂ concentration:

Cell No. at passage:

2.4x10^5 cells/ml

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Chromosome,

Chromosome,

(Date downloaded: 2008-08-27)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Inomata,M., Saijo,N., Kawashima,K., Kaneko,A., Fujiwara,Y., Kunikane,H., and Tanaka,Y. Induction of apoptosis in cultured retinoblastoma cells by the protein phosphatase inhibitor, okadaic acid, J. Cancer Res. Clin. Oncol., 121: 729-738, 1995.