| Profile:Mouse hybridoma cell line producing monoclonal antibody against human growth hormone (hGH). |
| Date accepted: | 10/12/2007 |
| Previous cell Number: | NIHS0556.16 |
| Animal: | mouse x mouse |
| Scientific Name: | hybridoma |
| Classification: | hybridoma |
| Histological type of the cell: | producing Mab human growth hormone (hGH). |
| Medium: | RPMI1640 medium with 10% fetal bovine serum. |
| Passage method: | dilution |
| CO2 Concentration: | 5% |
| Cell no. at passage: | split 1/5 (1/2 - 1/10) |
| Life span: | infinite |
| Morphology: | lymphocyte-like |
| Characteristics: | Producing IgG gamma1 and lambda |
| Established by | Sawada, J. |
| Deposited by | Sawada, J. |
| Cell bank: | NIHS(JCRB) |
| References: | (1) |
(* The format of the table was revised on Apr.25.2003.)
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No image(s) available. |
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No extradocuments available. |
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[ Lot No.: 06082009: seed (JCRB1222.16) ] |
Passage Number, p3* Cell culture was started without cloning from the previous stock. Date cell culture started:06/01/2009 Concentration of cells in an ampule: 3.5x10^6 cell/ml Viability under microscope (%): 87.9 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Anchorage dependency: No [ Culture condition of this lot.]
Freezing medium: Cell Banker BLC-1(Nihon Zenyaku Industries) Passage method: split 1/5(1/2-1/10) Growth temperature: 37 C CO2 concentration: 5 % Cell No. at passage: 1.1x10^5 cells/ml [ Additional Data ]
(Date downloaded: 2009-07-10) |