| Profile:Hybridoma cell line producing mAb HPSV178, against the C-terminal portion of PrPSV (residues 214-230). |
| Date accepted: | 07/01/2008 |
| Previous cell Number: | NIHS0596 |
| Animal: | mouse x mouse |
| Scientific Name: | hybridoma |
| Classification: | hybridoma |
| Date cell culture started: | 07/07/2008 |
| Medium: | RPMI1640 medium with 10% fetal bovine serum (100 uM Glutamine, 1 mM Sodium pyruvate and 50 uM 2-mercaptoetanol). |
| Passage method: | dilution |
| CO2 Concentration: | 5% |
| Cell no. at passage: | split 1/10 - 1/20 |
| Genetics: | Producing IgG2b(K) |
| Life span: | infinite |
| Morphology: | lymphocyte-like |
| Characteristics: | producing mAb HPSV178, against the C-terminal portion of PrPSV (residues 214-230). |
| Established by | Kikuchi, Y. |
| Deposited by | Kikuchi, Y. |
| Cell bank: | NIHS(JCRB) |
(* The format of the table was revised on Apr.25.2003.)
| Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Chromosome, Chromosome, |
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No extradocuments available. |
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[ Lot No.: 07152008: seed (JCRB1314) ] |
Passage Number, p2* Cell culture was started without cloning from the previous stock. Date cell culture started:07/07/2008 Concentration of cells in an ampule: 2.0x10^6 cell/ml Viability under microscope (%): 90.3 Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Kariology: mode = ND Chromosome frequency distribution: 41-58/5 62-81/4 92-95/14 96-99/24 >100/3 (50) Anchorage dependency: No [ Culture condition of this lot.]
Freezing medium: Cell Banker BLC-1(Nihon Zenyaku Industries) Passage method: 1/10-1/20 dilution Growth temperature: 37 C CO2 concentration: 5 % Cell No. at passage: 1.1x10^5 cells/ml [ Additional Data ]
(Date downloaded: 2009-03-11) |
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