JCRB1337:DL-40

JCRB1337 [DL-40]

Profile:Ki-1-positive T-cell lymphoma cell line.

Date accepted: 03/14/2007

Previous cell Number: NIHS0542

Animal: human

Sex and Age: F :64-year-old

Race: Japanese

Scientific Name: Homo sapiens

Case history: leukemic phase of non-Hodgkin's lymphoma

Classification: tumor

Histological type of the cell: lymphoma

Tissue prepared: peripheral blood

History of the cell: Kochi med. school -> JCRB cell bank.

Medium: RPMI 1640 medium with 10% fetal calf serum.

Passage method: Dilution.

CO₂ Concentration: 5%

Cell no. at passage: Split ratio = 1/2 -1/3.

Genetics: Cells are positive for acid phosphatase and negative for peroxidase, EBNA and HTLV-1 antigen.

Life span: infinite

Morphology: lymphoid

Characteristics: Cells grow without forming clumps with Dt=72 hrs.

Cell ID data: available

DNA Profile (STR): D5S818:9,12
D13S317:9,13
D7S820:10,11
D16S539:10,11
VWA:14,19
TH01:8
Amelogenin:X
TPOX:8,9
CSF1PO:13,14

Virus DNA detected: GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-)[-/negative, +/positive, nt/not tested,GAPDH is positive control] -- Description

Established by Kubonishi,I.

Deposited by Miyoshi,I.

Cell bank: NIHS(JCRB)

References: (1)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, STR-PCR(1), STR-PCR(2),

[ Extra document(s) ]

No extradocuments available.

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 05072009: seed (JCRB1337) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

75.4
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

RPMI1640 medium(GIBCO) with 10-20% heat inactivated fetal bovine serum(Hyclone GRD0051)

Freezing medium:

Cell Banker BLC-1(Nihon Zenyaku Industries)

Passage method:

Growth temperature:

CO₂ concentration:

Cell No. at passage:

7.0x10^5 cells/ml

[ Additional Data ]

Mycoplasma test,

STR-PCR(1),

STR-PCR(2),

(Date downloaded: 2012-02-01)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Kubonishi,I., Sonobe,H., Miyagi,T., Iwahara,Y., Ohyashiki,J.H., Ohyashiki,K., Toyama,K., Ohtsuki,Y., and Miyoshi,I. A Ki-1 (CD30)-positive T (E+, CD4+, Ia+)-cell line, DL-40, established from aggressive large cell lymphoma, Cancer Res., 50: 7682-7685, 1990.